离子束重组异常汉逊酵母菌Ar_Han0458的转录组学分析及谷胱甘肽代谢途径富集  

Transcriptomic analysis and glutathione metabolic pathway enrichment in recombinant Hansenula anomala Ar_Han0458

作　　者：张寒玉 唐朝 冯光文[1] 毛培宏[1] 蔡长龙[2] ZHANG Hanyu;TANG Chao;FENG Guangwen;MAO Peihong;CAI Changlong(Research Center of Radiation Ecology and Ion Beam Biotechnology,College of Physics Science and Technology,Xinjiang University,Urumqi 830046,China;Research Center of Ion Beam Biotechnology and Biodiversity,Xi’an Technological University,Xi’an 710032,China)

机构地区：[1]新疆大学物理科学与技术学院,放射生态与离子束生物技术中心,新疆乌鲁木齐830046 [2]西安工业大学,离子束生物工程与生物多样性研究中心,陕西西安710032

出　　处：《食品与发酵工业》2019年第10期10-15,共6页Food and Fermentation Industries

基　　金：国家自然科学基金项目(11575149; 31760016)

摘　　要：研究可稳定遗传的重组异常汉逊酵母Ar_Han0458在发酵过程中的差异基因表达信息及与谷胱甘肽代谢相关基因的表达变化。利用生物信息学方法分析重组异常汉逊酵母Ar_Han0458的5个发酵时间点的RNA-seq数据。重组异常汉逊酵母Ar_Han0458各发酵时间与0 h相比,下调表达基因数量多于上调表达;除发酵96和72 h外,相邻时间点的差异表达基因数目均以下调为主;发酵48和72 h时的基因表达谱相近。各发酵时间点间差异表达基因显著富集的GO功能均以细胞过程、细胞和催化活性为主。KEGG富集分析显示,10个差异表达基因参与了谷胱甘肽的代谢,谷胱甘肽的合成速率分别在24和96 h达到峰值,与实验测得胞外GSH产量变化趋势一致。重组异常汉逊酵母Ar_Han0458在发酵96 h时差异表达基因数目以上调为主,且谷胱甘肽合成速率最快。该研究为重组异常汉逊酵母的代谢调控研究及其分子育种提供了理论依据。This study aimed to investigate differential expression genes and changes in genes that are related to glutathione metabolism in a Hansenula anomala mutant, Ar_Han0458, during fermentation. The RNA-seq high-throughput sequencing data of Ar_Han0458 at five fermentation time points (0, 24, 48, 72, 96 h) were analyzed by bioinformatics methods. It was found that in comparison to 0 h, the number of down-regulated genes was more than that of up-regulated genes at each fermentation time. Except fermenting for 96 h and 72 h, the number of differential expression genes at adjacent time points was mainly down-regulated. Besides, the gene expression profiles were similar between 48 and 72 h. Moreover, the GO functions of differentially expressed genes at various time points mainly involved in cell process, cells and catalytic activity. The results of KEGG enrichment indicated that 10 differentially expressed genes participated in glutathione metabolism. The synthesis rate of glutathione reached the highest in 24 and 96 h, which was consistent with the variation trend of extracellular glutathione production. In conclusion, the number of differentially expressed genes in Ar_Han0458 was mainly up-regulated and it had the highest glutathione synthesis rate at 96 h. This study provides a theoretical basis for metabolic regulation of recombinant H. anomala and its molecular breeding.

关 键 词：重组异常汉逊酵母菌 转录组 差异表达基因 谷胱甘肽 代谢 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]