高分子质量聚唾液酸生产菌株诱变筛选及其发酵优化  

Mutagenesis and fermentation optimization of Escherichia coli K235 producing high molecular weight polysialic acid

作　　者：高霖 朱莉 杨泽林 詹晓北 吴剑荣 GAO Lin;ZHU Li;YANG Zelin;ZHAN Xiaobei;WU Jianrong(School of Biotechnology,Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education,Jiangnan University,Wuxi 214122,China;Wuxi Glyco Biotechology Co. Ltd,Wuxi 214125,China)

机构地区：[1]江南大学生物工程学院,糖化学与生物技术教育部重点实验室,江苏无锡214122 [2]无锡格莱克斯生物科技有限公司,江苏无锡214125

出　　处：《食品与发酵工业》2019年第10期22-28,共7页Food and Fermentation Industries

基　　金：863计划子课题(2012AA021505)

摘　　要：为获得产高分子质量聚唾液酸(polysialic acid,PSA)菌株,分步采用常温常压等离子体(atmospheric and room temperature plasma,ARTP)和硫酸二乙酯(diethyl sulfate,DES)对大肠杆菌K235进行诱变。结果表明,通过ARTP诱变筛选得到1株产PSA分子质量较初始菌株提高了36.84%的 E.coli K235 4B31。对 E.coli K235 4B31进行DES诱变,获得1株产PSA分子质量较初始菌株提高了78.18%的突变菌株 E.coli K235 6E61。通过三阶段搅拌转速控制策略最终使得突变菌株 E.coli K235 6E61发酵产物PSA分子质量达到430.5 kDa,为目前已报道的最高分子质量PSA。In order to produce polysialic acid (PSA) with high molecular weight, Escherichia coli K235 was treated by atmospheric and room temperature plasma (ARTP) and diethyl sulfate (DES). Mutant 4B31 obtained by ARTP mutagenesis produced PSA with 36.84% higher molecular weight than that of the parent strain. Mutant 6E61 obtained from mutant 4B31 by DES mutagenesis produced PSA with 78.18% higher molecular weight. By using a three-stage agitation speed fermentation, the maximal molecular weight of PSA produced by mutant 6E61 was 430.5 kDa, which was the highest value reported up to date.

关 键 词：大肠杆菌 聚唾液酸 高分子质量 发酵 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]