外源一氧化氮对低温胁迫下茶树幼苗生理特性的影响  

Effects of Exogenous Nitric Oxide on Physiological Characteristics of Tea Plants Under Cold Stress

作　　者：王英姿[1,2] 李银花 陈金华[1,2] 刘仲华 黄建安[1,2] WANG Yingzi;LI Yinhua;CHEN Jinhua;LIU Zhonghua;HUANG Jian’an(Key Laboratory of Tea Science of Ministry of Education,Hunan Agricultural University,Changsha 410128,China;National Research Center of Engineering & Technology for Utilization of Functional Ingredients from Botanicals,Hunan Agricultural University,Changsha 410128,China)

机构地区：[1]湖南农业大学教育部茶学重点实验室,湖南长沙410128 [2]国家植物功能成分利用工程技术研究中心,湖南长沙410128

出　　处：《茶叶科学》2019年第3期335-341,共7页Journal of Tea Science

基　　金：湖南省教育厅科学研究项目(17C0767);校科学基金项目(16QN09)

摘　　要：为探究外源一氧化氮(Nitric oxide,NO)供体硝普钠(Sodium nitroprusside,SNP)对低温胁迫下茶树幼苗的缓解作用,采用人工气候室模拟低温胁迫试验,研究了叶面喷施不同浓度SNP对低温胁迫下两年生碧香早茶苗叶片膜脂过氧化程度、渗透调节物质含量及抗氧化酶活性的影响。结果表明,适宜SNP浓度可降低低温胁迫下茶树叶片相对电导率,抑制丙二醛含量的升高,促进脯氨酸、可溶性蛋白和可溶性糖的大量积累,提高超氧化物歧化酶、过氧化氢酶活性,从而缓解低温胁迫对茶树叶片的伤害。试验结果表明,喷施浓度为200μmol·L^-1的SNP效果最佳。In order to investigate the alleviation effect of exogenous nitric oxide (NO) donor sodium nitroprusside (SNP) on tea rooted cuttings under cold stress, artificial climate chamber was used to simulate cold stress on tea rooted cuttings. The effects of SNP on membrane lipid peroxidation, osmotic adjustment substance and antioxidant activity of biennial ‘Bixiangzao’ rooted cuttings under cold stress were studied by foliar spray of SNP. The results show that suitable SNP concentration treatment could reduce the relative conductivity of tea leaves, inhibit the elevated malondialdehyde content, promote the accumulation of proline, soluble protein and sugar, and increase the superoxide dismutase, catalase activities, thereby alleviate the damage of cold stress on tea leaves. In addition, the optimal concentration of SNP was 200 μmol L^-1.

关 键 词：茶树 低温胁迫 外源NO 硝普钠 生理特性 

分 类 号：S571.1[农业科学—茶叶生产加工] S339.4[农业科学—作物学]