miR-21对肝癌细胞Keap-1/Nrf2通路的调控作用分析  

The regulation of miR-21 on the Keap-1/Nrf2 pathway in hepatoma cells

作　　者：徐志诚[1] 陈晓宁[2] 李春海[1] 王晓东[1] 李准[1] XU Zhi-cheng;CHEN Xiao-ning;LI Chun-hai(Department of General Surgery,Jiamusi Center Hospital,Jiamusi Heilongjiang 154002,China;Department of General Surgery,The 2nd Affiliated Hospital of Harbin Medical University,Harbin Heilongjiang 150086,China.)

机构地区：[1]佳木斯市中心医院普外科,黑龙江佳木斯154002 [2]哈尔滨医科大学附属第二医院普通外科,黑龙江哈尔滨150086

出　　处：《临床和实验医学杂志》2019年第11期1141-1144,共4页Journal of Clinical and Experimental Medicine

基　　金：黑龙江省青年科学基金项目(编号:QC2013C112)

摘　　要：目的探讨miR-21对肝癌细胞Keap-1/Nrf2通路的调控作用。方法人肝癌细胞株HSMMC-7721随机分为三组,实验组、对照组分别转染miRNA-21模拟剂(mimics)、miRNA-21 NC,空白组不进行转染。采用MTT法检测细胞增殖,流式细胞仪实验检测细胞周期,Transwell小室实验检测细胞侵袭,Western blot检测蛋白表达。结果转染后36 h与48 h,实验组的miRNA-21相对表达量显著高于空白组和对照组(P<0.05),实验组的细胞增殖率、侵袭率显著低于空白组和对照组(P<0.05);转染后48 h,实验组的S期与G2/M期比率显著高于空白组和对照组,(P<0.05),GO/G1期比率显著低于空白组和对照组,(P<0.05),空白组和对照组对比差异无统计学意义(P>0.05)。转染后36 h与48 h,与空白组和对照组相比,实验组的Keap-1、Nrf2蛋白相对表达量显著下降(P<0.05)。结论miRNA-21高表达能抑制Keap-1/Nrf2通路的激活,可调节肝癌细胞周期,从而抑制肝癌细胞的增殖与侵袭。Objective To investigate the regulation of miR-21 on the Keap-1/Nrf2 pathway in hepatoma cells.Methods Human hepatoma cell line HSMMC-7721 were randomly divided into three groups.The experimental group and the control group were transfected with miRNA-21 mimics and miRNA-21 NC,respectively.The blank group were not transfected.Cell proliferation were detected by MTT assay.Flow cytometry was used to detect the cell cycle,cell invasion were detected by Transwell chamber assay,and protein expression were detected by Western blot.Results At 36 h and 48 h after transfection,the relative expression of miRNA-21 in the experimental group were significantly higher than that in the blank group and the control group(P<0.05).The cell proliferation rate of the experimental group were significantly lower(P<0.05).The invasive rates were significantly decreased(P<0.05);at 48 hours after transfection,the proportion of S and G2/M phases in the experimental group were significantly increased(P<0.05),and the proportion of GO/G1 phase were significantly decreased(P<0.05).There were no significant difference in the control group(P>0.05).At 36 h and 48 h after transfection,the relative expression of Keap-1 and Nrf2 protein in the experimental group were significantly lower than those in the blank group and the control group(P<0.05).Conclusion High expression of miRNA-21 can inhibit the activation of Keap-1/Nrf2 pathway,regulate the cell cycle of liver cancer,and inhibit the proliferation and invasion of liver cancer cells.

关 键 词：肝癌 MIR-21 Keap-1/Nrf2通路 细胞周期 

分 类 号：R735.7[医药卫生—肿瘤]