微柱萃取与高效液相色谱联用测定镇定剂残留  

Determination of Sedative Residues by Combining Micropillar Extraction with High Performance Liquid Chromatography

作　　者：张素玲[1] 徐煜锋 王冠杰 张春晓[1] 华子泺 ZHANG Suling;XU Yufeng;WANG Guanjie;ZHANG Chunxiao;HUA Ziluo(School of Materials & Encironmental Engineering,Hangzhou Dianzi Unicersity,Hangzhou Zhejiang 310018,China)

机构地区：[1]杭州电子科技大学材料与环境工程学院,浙江杭州310018

出　　处：《杭州电子科技大学学报（自然科学版）》2019年第3期73-77,共5页Journal of Hangzhou Dianzi University：Natural Sciences

基　　金：浙江省科技计划资助项目(2017C37041)

摘　　要：环境水和血液样品中镇静剂残留浓度低,且基质复杂,干扰严重,需经高效样品前处理技术对目标物进行富集分离。采用C_(18)微柱离心萃取解吸的新型微萃取模式,与高效液相色谱联用,建立了环境水和血样中三唑仑、安定和唑吡坦3种镇静剂残留的分析方法。研究结果表明,NaCl浓度为0.35 g/mL条件下萃取效果最佳。在最佳条件下,3种镇定剂在20～200μg/L范围内与峰面积呈良好的线性关系,相关系数均大于0.99。三唑仑、安定和唑吡坦的检出限分别为2.51μg/L,2.67μg/L和3.36μg/L。用于河水和血液样品中镇定剂残留测定,平均加标回收率高于84%。Due to low trace mount of sedative residues and the complicated matrix of environmental water and blood,it is necessary to develop an efficient sample pretreatment process for the enrichment and separation of target analytes prior to analysis.A new solid phase microextraction model based on the combination between C18 micropillar and high performance liquid chromatography(HPLC)is used for the determination of triazolam,diazepam and zolpidem in environmental water and blood samples.The experimental result shows that the best extraction efficiency is obtained under 0.35 g/mL NaCl.Under the optimum condition,the linear range of the three sedatives is 20~200μg/L,with the correlation coefficients(R2)greater than 0.99.The detection limits of triazolam,diazepam and zolpidem are 2.51μg/L,2.67μg/L and 3.36μg/L respectively.The method is applied for the determination of sedative residues in river water and blood samples,and the average recovery rates are higher than 84%.

关 键 词：镇静剂 C18微柱 固相微萃取 高效液相色谱 

分 类 号：O652.6[理学—分析化学]