miR-21在成人急性B淋巴细胞白血病患者中的表达及临床意义  

Expression and clinical significance of microRNA-21 in patients with acute B-lymphoblastic leukemia

作　　者：王田园 蔡安季[1] 郝亚军 刘诗诗 李钢[1] 谭文 郑宝 WANG Tian-yuan;CAI An-ji;HAO Ya-jun;LIU Shi-shi;LI Gang;TAN Wen;ZHENG Bao(Department of Laboratory,Nanshan People's Hospital,Shenzhen 518052,Guangdong,CHINA)

机构地区：[1]深圳市南山区人民医院检验科

出　　处：《海南医学》2019年第11期1375-1378,共4页Hainan Medical Journal

摘　　要：目的探讨miR-21在成人急性B淋巴细胞白血病(B-ALL)患者中的表达特点,分析其与临床治疗、预后的关系。方法选择2016年1月至2018年12月深圳市南山区人民医院血液科收治的48例B-ALL患者纳入研究组,并选择40例骨髓正常的非造血系统疾病患者作为对照组,所有受试者均采集骨髓标本采用实时荧光定量PCR法检测骨髓单核核细胞(BMMCs)中miR-21表达情况并进行比较。研究组患者均接受VDLP方案化疗2个疗程共2个月,比较高危组及标危组患者的miR-21表达的差异,根据化疗结果将患者分为耐药组和敏感组,比较组间miR-21表达差异,分析miR-21表达与B-ALL患者微小残留病(MRD)的相关性。结果研究组患者的miR-21表达量为7.02±0.53,明显高于对照组的2.43±0.27,差异有统计学意义(P<0.05);高危组患者的miR-21表达量为8.72±0.67,明显高于标危组的5.62±0.41,差异有统计学意义(P<0.05);治疗后研究组患者miR-21表达量为5.03±0.35,较治疗前明显下降,差异有统计学意义(P<0.05),其中敏感组患者的miR-21表达量为4.24±0.27,明显低于耐药组的6.33±0.38,差异有统计学意义(P<0.05);相关性分析结果显示miR-21相对表达量与MRD呈负相关(r=-0.432,P<0.05)。结论成人B-ALL患者miR-21表达明显升高,miR-21表达与患者的临床治疗疗效、预后有着密切的关系。Objective To investigate the expression of microRNA-21 in adult patients with acute B-lymphoblastic leukemia(B-ALL), and to analyze its relationship with clinical treatment and prognosis. Methods From January 2016 to December 2018, 48 patients with B-ALL admitted to Department of Hematology, Nanshan People’s Hospital were selected as the study group, and 40 patients with normal and non-hematopoietic diseases of bone marrow were selected as control group. Bone marrow samples were collected from all subjects and the expression of microRNA-21 in bone marrow mononuclear cells(BMMCs) was detected by real-time fluorescence quantitative PCR. The expression of microRNA-21 between high-risk group and standard-risk group were compared. Patients with B-ALL were treated with VDLP regimen for two courses(2 months). According to the results of chemotherapy, patients were divided into drug-resistant group and sensitive group. The expression of microRNA-21 was compared between the two groups, and the correlation between microresidual disease(MRD) and the expression of microRNA-21 was analyzed. Results The expression of microRNA-21 in the study group was 7.02±0.53, which was significantly higher than 2.43±0.27 in the control group(P<0.05). The expression of microRNA-21 in high-risk group was 8.72±0.67, which was significantly higher than5.62 ± 0.41 in standard-risk group(P<0.05). After treatment, the expression of microRNA-21 in B-ALL patients was5.03 ± 0.35, which was significantly lower than that before treatment(P<0.05). The expression of microRNA-21 in B-ALL patients in sensitive group was 4.24 ± 0.27, which was significantly lower than 6.33 ± 0.38 in the drug-resistant group(P<0.05). The correlation analysis showed that the relative expression of microRNA-21 was negatively correlated with MRD(r=-0.432, P<0.05). Conclusion The expression of microRNA-21 in adult B-ALL patients is significantly increased. The expression of microRNA-21 is closely related to the clinical efficacy and prognosis of B-ALL.

关 键 词：MIR-21 急性B淋巴细胞白血病 预后 实时定量PCR 白血病 微小残留病 

分 类 号：R733.71[医药卫生—肿瘤]