siRNA沉默CD44表达对人胆囊癌细胞增殖、侵袭和迁移的影响  

Effects of CD44 on proliferation, migration and invasion of human gallbladder carcinoma cells

作　　者：蔡炜龙[1] 余胜[1] 汪伟民[1] 许洪宝[1] 楼能 CAI Weilong;YU Sheng;WANG Weimin(Department of Surgery,Huzhou Central Hospital,Huzhou 313000,China)

机构地区：[1]湖州市中心医院普通外科

出　　处：《浙江医学》2019年第11期1133-1137,共5页Zhejiang Medical Journal

基　　金：浙江省医药卫生科技计划项目平台计划(2019ZD051)

摘　　要：目的探讨siRNA沉默CD44表达对人胆囊癌细胞增殖、侵袭和迁移的影响。方法利用RNA干扰技术在人胆囊癌细胞株EH-GB1和GBC-SD中敲低CD44的表达;通过CCK8实验检测si-NC和si-CD44对人胆囊癌细胞株EH-GB1和GBC-SD增殖能力的影响;通过Transwell迁移实验检测其对迁移能力的影响;通过Transwell侵袭实验检测其对侵袭能力的影响。结果转染si-CD44对胆囊癌细胞GBC-SD和EH-GB1的增殖能力无明显影响(P>0.05),但能明显抑制胆囊癌细胞的迁移能力[si-NC组穿膜细胞数在人胆囊癌细胞株EH-GB1、GBC-SD分别为(193.7±3.9)、(193.7±7.8)个,明显大于si-CD44组的(88.7±5.8)、(110.3±5.5)个(t=15.12、10.42,均P<0.05)]和侵袭能力[(si-NC组穿膜细胞数在人胆囊癌细胞株EH-GB1、GBC-SD分别为(175.3±7.5)、(188.0±9.0)个,明显大于si-CD44组的(85.3±3.7)、(73.0±3.6)个(t=10.74、11.84,均P<0.05)]。结论CD44能够促进胆囊癌细胞的侵袭、迁移能力,可能作为胆囊癌治疗的生物靶点。Objective To investigate the effects of CD44 gene upon the proliferation,migration and invasion of human gallbladder carcinoma cells.Methods CD44 expression in gallbladder cancer EH-GB1 and GBC-SD cells was silenced by si RNA.The proliferation of EH-GB1 and GBC-SD cells was detected by CCK8 assay;cell migration ability and invasion ability were detected by Transwell migration assay.Results Knockdown of CD44 had no significant effect on the proliferation of gallbladder cancer EH-GB1 and GBC-SD cells(P>0.05).While knockdown of CD44 significantly inhibited the migration and invasion of EH-GB1 and GBC-SD cells.Transwell cell migration assay revealed that the numbers of membrane-penetrating cells at 24 h in the si-NC groups of EH-GB1 and GBC-SD cells were(193.7±3.9)and(193.7±7.8),which were significantly more than those of si-CD44 groups(88.7±5.8,t=15.12,P<0.05)and(110.3±5.5,t=10.42,P<0.05).Transwell cell invasion assay revealed that the numbers of membrane-penetrating cells at 24 h in the si-NC group were(175.3±7.5)and(188.0±9.0),which were significantly more than those in si-CD44 control groups(85.3±3.7,t=10.74,P<0.05)and(73.0±3.6,t=11.84,P<0.05).Conclusion CD44 can promote the invasion and migration of gallbladder cancer cells,indicating that it might be a therapeutic target for the prevention and treatment of gallbladder cancer.

关 键 词：胆囊肿瘤 CD44 SIRNA 增殖 侵袭 迁移 

分 类 号：R735.8[医药卫生—肿瘤]