云南汉族人群HLA-DM基因多态性与HCV慢性感染的相关性分析  

Association Analysis between of HLA-DM Polymorphisms and Chronic HCV Infection in Yunnan Han Population

作　　者：陈珺 杨瑾 刘楠楠 刘舒媛[1] 李传印[1] 张新文[1] 史荔[1] 张淑琼 CHEN Jun;YANG Jin;LIU Nannan;LIU Shuyuan;LI Chuanyin;ZHANG Xinwen;SHI Li;ZHANG Shuqiong(Yunnan Key Laboratory of Vaccine Development on Severe Infectious Disease,Institute of Medical Biology,Chinese Academy of Medical Sciences & Peking Union Medical College,Kunming 650118,Yunnan,China;The Third People's Hospital in Yunnan Province,Kunming 650041,Yunnan,China)

机构地区：[1]中国医学科学院&北京协和医学院医学生物学研究所云南省重大传染病疫苗研发重点实验室,云南昆明650118 [2]昆明市第三人民医院,云南昆明650041

出　　处：《贵州医科大学学报》2019年第5期524-529,共6页Journal of Guizhou Medical University

基　　金：国家自然科学基金(31570918);中国医学科学院医学与健康科技创新工程项目(2016-I2M-2-001)

摘　　要：目的:研究云南汉族人群中HLA-DM基因多态性与丙型肝炎病毒(HCV)慢性感染的关系。方法:选取云南汉族人群HCV慢性感染者185人(病例组),健康对照180人(对照组),采用Sanger测序法和TA克隆测序法对HLA-DMA,HLA-DMB基因进行基因分型,计算这两个基因在病例组和对照组中的等位基因频率和基因型频率;构建单倍型,比较两组间的分布差异。结果:在病例组和对照组中,共检出4种HLA-DMA等位基因和5种HLA-DMB等位基因,DMA*01∶01-DMB*01∶03单倍型在病例组中的频率显著高于对照组(0.287vs0.197,OR=0.134,95%CI为1.529~2.305,P=0.005),DMA*01∶02-DMB*01∶03单倍型在病例组中的频率显著低于对照组(0.024vs0.056,OR=0.424,95%CI为0.194~0.944,P=0.031)。结论:单倍型DMA*01∶01-DMB*01∶03和DMA*01∶02-DMB*01∶03可能与云南汉族人群HCV慢性感染相关。Objective:To investigate the association between HLA-DM gene polymorphism and chronic hepatitis C virus(HCV)infection in Yunnan Han population.Methods:We recruited 185 patients with chronic HCV infection(case group)and 180 healthy controls(control group)in Yunnan Han population.HLA-DMA and HLA-DMB genes were genotyped by Sanger sequencing and TA clone sequencing.Allele frequencies and genotype frequencies were calculated.In addition,haplotypes were constructed to compare the distribution frequency.Results:Four HLA-DMA alleles and five HLA-DMB alleles were detected in the both groups.The DMA*01∶01-DMB*01∶03 haplotype was significantly higher in the case group than that in the control group(0.287 vs 0.197,OR=0.134,95%CI was 1.529~2.305,P=0.005),while the DMA*01∶02-DMB*01∶03 haplotype was significantly lower in the case group than that in control group(0.024 vs 0.056,OR=0.424,95%CI was 0.194~0.944,P=0.031).Conclusion:DMA*01∶01-DMB*01∶03 and DMA*01∶02-DMB*01∶03 haplotypes may be associated with chronic HCV infection in Yunnan Han population.

关 键 词：HLA-DM 肝炎 丙型 慢性感染 云南 汉族 基因多态性 等位基因 

分 类 号：R512.63[医药卫生—内科学]