GOLPH3在6种肿瘤中的表达特点  

The Expression of GOLPH3 in Six Kinds of Tumors

作　　者：于莉娜[1,2] 田燕[1,2] 赵佩佩 翁雅倩 刘桢 华兴 YU Lina;TIAN Yan;ZHAO Peipei;WENG Yaqian;LIU Zhen;HUA Xing(Department of Pathology,Nanfang Hospital,Southern Medical University,Guangzhou 510515,Guangdong,China;Department of Pathology,School of Basic Medical Sciences,Southern Medical University,Guangzhou 510515,Guangdong,China;Department of Pathology,School of Clinical Medical Sciences,Guizhou Medical University,Guiyang 550004,Guizhou,China;Department of Pathology,Guangzhou Red Cross HospitalAffiliated to Medical College of Jinan University,Guangzhou 510220,Guangdong,China)

机构地区：[1]南方医科大学南方医院病理科,广东广州510515 [2]南方医科大学基础医学院病理学系,广东广州510515 [3]贵州医科大学临床医学院病理学教研室,贵州贵阳550004 [4]暨南大学医学院附属广州红十字会医院,广东广州510220

出　　处：《贵州医科大学学报》2019年第5期530-535,共6页Journal of Guizhou Medical University

基　　金：国家自然科学基金(81472319);广东省自然科学基金(2014A030310134);广州市科技计划项目(201707010465)

摘　　要：目的:探讨反面高尔基网的癌基因GOLPH3在人胃腺癌(STAD)、肝细胞癌(HCC)、乳腺癌(BRCA)、子宫内膜癌(UCEC)、睾丸生殖细胞肿瘤(TGCT)及前列腺癌(SART)组织中的表达特点。方法:应用生物信息技术对GOLPH3的肿瘤组织分布情况进行分析,筛选出GOLPH3表达上调癌组织-STAD、BRCA、UCEC及TGCT;应用免疫组织化学SP法分别检测人STAD、HCC、BRCA、UCEC、TGCT及SART组织(n=30)及其癌旁组织(n=10)中GOLPH3蛋白的表达水平,采用逆转录定量PCR(qRT-PCR)技术检测人STAD细胞株、人HCC细胞株、人BRCA细胞株、人SART细胞株及其相对应的对照细胞株中GOLPH3mRNA表达水平,分析GOLPH3表达水平与恶性肿瘤的关系。结果:GOLPH3广泛表达于多个人体正常组织,在STAD、HCC、BRCA、UCEC、TGCT、SART肿瘤细胞中表达显著上调;免疫组织化学及qRT-PCR结果显示,GOLPH3的蛋白及mRNA在相应实体恶性肿瘤组织及细胞株中的表达水平较癌旁组织或对照细胞的表达水平显著升高,差异具有统计学意义(P<0.01)。结论:GOLPH3表达上调可能在STAD、BRCA、UCEC、TGCT、HCC及SART的发生发展过程中发挥重要促进作用。Objective:To investigate expression of GOLPH3,an oncogene of the reverse Golgi reticulum,in human gastric adenocarcinoma(STAD),hepatocellular carcinoma(HCC),breast cancer(BRCA),endometrial cancer(UCEC),testicular germ cell tumor(TGCT)and prostate cancer(SART).Methods:The distribution of GOLPH3 in cancer tissues was analyzed by using bioinformatics technology,and the up-regulated expression of GOLPH3 in cancer tissues(STAD,BRCA,UCEC and TGCT)was screened out.The expression levels of GOLPH3 protein in human STAD,HCC,BRCA,UCEC,TGCT and SART tissues and their adjacent tissues were detected by immunohistochemical SP method.The expression of GOLPH3 in human STAD cell lines,human HCC cell lines,human BRCA cell lines,human SART cell lines and their corresponding control cell lines were detected by quantitative reverse transcription polymerase chain reaction(qRT-PCR).The relationship between GOLPH3 expression and malignant tumors was analyzed.Results:GOLPH3 was widely expressed in many human normal tissues,and up-regulated in STAD,HCC,BRCA,UCEC,TGCT and SART tumor cells.Immunohistochemical and qRT-PCR results showed that the expression level of GOLPH3 protein and its mRNA in the corresponding solid malignant tumor tissues and cell lines was significantly higher than that in adjacent tissues or control cells(P<0.01).Conclusion:Upregulation of GOLPH3 expression may play an important role in the occurrence and development of STAD,BRCA,UCEC,TGCT,HCC and SART.

关 键 词：GOLPH3 肿瘤 生物信息学 免疫组织化学 QRT-PCR 分子标志物 

分 类 号：R361.2[医药卫生—病理学]