小麦食源性过敏原致敏亚基分析  

Analysis of Allergenic Subunits for Wheat Foodborne Allergens

作　　者：姚亚亚 刘阳星月 李宇凡 苗字叶 刘壮[1] 李慧静[1,2] Yao Yaya;Liu Yangxingyue;Li Yufan;Miao Ziye;Liu Zhuang;Li Huijing(College of Food Science and Techology,Agricultural University of Hebei,Baoding 071001;Beijing Advanced Innovation Center for Food Nutrition and Human Health,Beijing Technology & Business University,Beijing 100048)

机构地区：[1]河北农业大学食品科技学院,保定071001 [2]北京食品营养与人类健康高精尖创新中心,北京工商大学,北京100048

出　　处：《中国粮油学报》2019年第5期140-146,共7页Journal of the Chinese Cereals and Oils Association

基　　金：河北省二期现代农业小麦产业技术体系创新团队产后加工与食品开发(HBCT2018010207);北京食品营养与人类健康高精尖创新中心开放基金(20161008)

摘　　要：通过SDS-PAGE对小麦亚基成分分析,采用ImmunoCAP250全自动体外免疫检测系统测定小麦过敏患者不同个体对小麦致敏蛋白的识别结果,并以我国12例小麦过敏患者的血清为探针,3例正常人的血清池为对照,与师栾02-1小麦粉进行还原态一维电泳免疫印迹分析,鉴定引起我国小麦过敏人群过敏的小麦过敏原及其致敏概率。结果表明,小麦过敏原蛋白亚基的致敏概率自高至低的顺序依次为17ku(6/12)、28ku(6/12)、14ku(5/12)、19ku(5/12)、22ku(5/12)、24ku(5/12)、25ku(5/12)、26ku(5/12)、11ku(4/12)、12ku(4/12)、16ku(4/12)、21ku(4/12)、27ku(4/12)、18ku(3/12)、30ku(3/12)、31ku(3/12)、36ku(3/12)、47ku(3/12)、83ku(3/12),其他一些蛋白亚基致敏概率为1/6或1/12。In this paper, the immune CAP-250 automatic in vitro immunoassay system was used to determine the identification results of allergenic wheat protein in wheat allergy patients through the analysis of the subunits of wheat by SDS-PAGE. And the sera of 12 wheat allergy patients in China were used as the probes and the serum pool of 3 negative persons was used as the control. And the wheat allergens and allergenicity for allergic people of China were identified by the one-dimensional electrophoretic western blotting analysis of Shiluan No.02-1 wheat flour. The results showed that the allergenic probabilities of wheat allergenic protein subunits were 17kD (6/12), 28ku (6/12), 14ku (5/12), 19ku(5/12), 22ku (5/12), 24 ku (5/12), 25 ku (5/12), 26 ku (5/12), 11 ku (4/12), 12 ku (4/12), 16 ku (4/12), 21 ku (4/12), 27ku (4/12), 18ku (3/12), 30ku (3/12), 31ku (3/12), 36ku (3/12), 47ku (3/12), 83ku (3/12), the probability of some other allergenic protein subunits was 1/6 or 1/12.

关 键 词：小麦亚基 过敏原 致敏概率 免疫印迹分析 

分 类 号：TS201.6[轻工技术与工程—食品科学]