miR-24在胆管癌组织和细胞系HCCC-9810中的表达  

Expression of miR-24 in cholangiocarcinoma tissues and cell line HCCC-9810

作　　者：窦雯雯[1] 孙广洪[1] 杨敏[1] 谢瑞柱 苏艳 徐建华 赵雪 DOU Wen-wen;SUN Guang-hong;YANG Min;XIE Rui-zhu;SU Yan;XU Jian-hua;ZHAO Xue(Department of Infectious Diseases,Affiliated Hospital of Weifang Medical University,Weifang 261000,China)

机构地区：[1]潍坊医学院附属医院感染性疾病科

出　　处：《基础医学与临床》2019年第6期816-820,共5页Basic and Clinical Medicine

基　　金：山东省医药卫生科技发展计划项目(2017WS849,2017WS777)

摘　　要：目的探讨microRNA-24(miR-24)在胆管癌患者组织中的表达情况,并分析其表达预后价值及生物学功能。方法收集124例胆管癌患者的癌组织和癌旁组织标本,用实时荧光定量PCR检测miR-24含量,使用χ~2检验对miR-24和胆管癌临床病理学特征的关系进行分析,并通过Kaplan-Meier生存曲线和Cox回归模型评估miR-24在胆管癌中的预后价值。以人胆管癌细胞系HCCC-9810为研究对象,通过转染pEGFP-C1-miR-24融合表达载体,分析miR-24对胆管癌细胞增殖、迁移和侵袭能力的影响。结果 miR-24在胆管癌组织中的表达较癌旁组织上调(P<0.05),且与患者淋巴结转移、肿瘤细胞分化及TNM分期显著相关。miR-24高表达的胆管癌患者的整体生存率显著低于低表达患者(P<0.001),是胆管癌的独立预后因子(HR=5.518, 95%CI=2.394~12.717,P<0.001)。此外,胆管癌细胞中上调的miR-24能够显著地促进癌细胞的增殖、迁移和侵袭的能力。结论胆管癌中表达水平上调的miR-24与患者不良预后显著相关,并参与了胆管癌的进展过程,可能成为胆管癌治疗的新靶点。Objective To explore the expression patterns of microRNA-24(miR-24) in cholangiocarcinoma(CCA) tissues, and to analyze its prognostic value and biological function in HCC. Methods A total of 124 pairs of CCA tissues and adjacent tissues were collected, and the expression of miR-24 in the tissues was estimated by quantitative real-time qPCR(RT-qPCR).χ~2 test was used to assess the association of miR-24 with clinicopathological characteristics of the patients. The prognostic value of miR-24 was evaluated by Kaplan-Meier survival curves and Cox regression model. The effect of miR-24 on cell proliferation, migration and invasion was investigated in CCA cell line HCCC-9810 by cell transfection with fusion expression vector pEGFP-C1-miR-24. Results The expression of miR-24 was significantly upregulated in CCA tissues compared with the paired noncancerous tissues(P<0.05), and was associated with lymph node metastasis, differentiation and TNM stage of the patients. The patients with high miR-24 expression had poor overall survival as compared with those with low miR-24 expression(log-rank P<0.001). The expression of miR-24 was considered an independent prognostic factor(HR=5.518, 95% CI=2.394-12.717, P<0.001). In addition, the overexpression of miR-24 in CCA cells led to enhanced cell prolifera-tion, migration and invasion. Conclusions The upregulated expression of miR-24 is associated with poor prognosis of CCA and involved in tumor progression, which may be a novel and potential target for CCA treatment.

关 键 词：miR-24 胆管癌 预后 增殖 转移 

分 类 号：R73-3[医药卫生—肿瘤]