转录因子ZBTB18对结直肠癌细胞转移的影响  

Effects of transcription factor ZBTB18 on colorectal cancer cell metastasis

作　　者：田力 刘波 彭朝胜 夏菁 吕贯廷 TIAN Li;LIU Bo;PENG Chaosheng;XIA Jing;LV Guanting(Department of Special Medicine,the Sixth Medical Center,Chinese PLA General Hospital,Beijing 100048,China;Beijing Institute of Genomics,Chinese Academy of Sciences,Beijing 100101,China;Department of Blood Transfusion,Tangdu Hospital,Air Force Medical University,Xi’an Shaanxi 710038,China)

机构地区：[1]中国人民解放军总医院第六医学中心特需医疗部,北京100048 [2]中国科学院北京基因组研究所,北京100101 [3]空军军医大学唐都医院输血科,陕西西安710038

出　　处：《转化医学杂志》2019年第3期140-143,共4页Translational Medicine Journal

基　　金：国家自然科学基金(81101490)

摘　　要：目的 观察转录因子ZBTB18对结直肠癌细胞的生长的影响。方法 从NCBI GEO数据库中下载肿瘤基因表达芯片数据,对ZBTB18基因的表达水平进行分析;同时通过RNA干扰和Transwell实验研究ZBTB18对结直肠癌细胞迁移的影响。结果 ZBTB18蛋白表达于结直肠癌细胞系,且高转移能力的细胞系中该蛋白的表达水平远高于原发性结直肠癌肿瘤细胞系( P <0.05)。RNA干扰试验可特异性地显著沉默结直肠癌细胞HT29中ZBTB18的表达,Transwell实验发现在ZBTB18沉默后HT29细胞的侵袭和迁移能力显著降低( P <0.05)。结论 转录因子ZBTB18可以促进结直肠癌细胞的侵袭与迁移,可能参与结直肠癌的转移。Objective To study the effect of ZBTB18 on the colorectal cancer cell growth.Methods The expression profiling data of colorectal cancer was downloaded from the public database NCBI GEO and the expression of ZBTB18 was analyzed specifically. We detected ZBTB18 expression levels by quantitative PCR in multiple colorectal cancer cell lines,reduced its expression level by RNAi and observed the cell invasion and migration by Transwell experiment. Results The expression level of ZBTB18 in metastatic tumor cells was higher than that in non-metastatic tumor cells( P<0. 05). After reducing the expression of ZBTB18 in HT29 cells by RNAi,we found the down expression of ZBTB18 could significantly inhibit the cell invasion and migration in vitro( P <0. 05). Conclusion Transcription factor ZBTB18 could promote the invasion and migration of colorectal cancer cells,suggesting that ZBTB18 may promote colorectal cancer metastasis.

关 键 词：结直肠癌 ZBTB18 转移 

分 类 号：R735.34[医药卫生—肿瘤]