miR-337-3p对胃癌细胞SGC7901中HPSE表达的调控及其对细胞增殖的影响  

MiR-337-3p is involved in proliferation in gastric cancer cells SGC7901 via regulating HPSE

作　　者：潘国友 董康迪 谢德天 彭利盼[2] PAN Guo-you;DONG Kang-di;XIE De-tian;PENG Li-pan(Ward 4 of Gynaecological Tumors,Shandong Institute of cancer prevention and control(Jinan 250117,China);Department of Gastrointestinal Surgery,Shandong Provincial Hospital affilicated to Shandong Universtity(Jinan 250021,China))

机构地区：[1]山东省肿瘤防治研究院妇科肿瘤四病区,山东济南250017 [2]山东大学附属省立医院胃肠外科,山东济南250021

出　　处：《中国现代普通外科进展》2019年第4期259-261,265,共4页Chinese Journal of Current Advances in General Surgery

基　　金：山东省自然科学基金(ZR2015PH024);山东省重点研发计划项目(2017GSF221003

摘　　要：目的:探讨miR-337-3p对胃癌细胞SGC7901中HPSE表达的调控作用及其对细胞增殖的影响。方法:miR-337-3p mimics转染SGC7901细胞后,应用RT-PCR、Western blot检测其对miR-337-3p表达水平的影响及其对HPSE表达的影响;荧光素酶实验检测转染细胞中HPSE 3’-UTR荧光素酶活性;CCK-8测定细胞增殖的影响。结果:上调miR-337-3p可以抑制HPSE的表达、降低HPSE 3’-UTR启动子荧光素酶活性,并可抑制胃癌细胞增殖。结论:miR-337-3p可以通过下调HPSE的表达抑制胃癌细胞SGC7901增殖。Objective: To explore the mechanism of miR-337-3p regulating the expression of HPSE and the effect of proliferation of gastric cancer cells SGC7901. Methods: Mi R-337-3p mimics were transfected into SGC7901 cells,expression of miR-337-3p and HPSE were detected by RT-PCR and Western blot.Dual luciferase reporter assay was used to detect the target relationship between miR-337-3p and HPSE 3’-UTR promoter.Then cell proliferation was detected by CCK-8 assay. Results: Exogenous expression of miR-337-3p could inhibit the expression of HPSE and luciferase activity of HPSE 3’-UTR promoter.Upregulation of miR-337-3p also inhibited cell proliferation of SGC7901 cells. Conclusion: Mi R-337-3p is potentially involved in proliferation in gastric cancer cells SGC7901 via its regulating the expression of HPSE.

关 键 词：MiR-337-3p HPSE 胃癌 增殖 

分 类 号：R735.2[医药卫生—肿瘤]