脑源性神经营养因子基因和神经营养性酪氨酸激酶受体Ⅱ型基因交互作用与青年自杀未遂行为的关系  

Association of interaction between brain-derived neurotrophic factor gene and neurotrophic tyrosine kinase receptor type 2 gene with suicide attempt in middle and later adolescents

作　　者：吴同 冉柳毅 靳佳佳[2] 王苏亚[2] 刘微 艾明[2] 陈建梅[2] 况利[2] WU Tong;RAN Liuyi;JIN Jiajia;WANG Suya;LIU Wei;Al Ming;CHEN Jianmei;KUANG LI(Center for Mental Health,University-Town Hospital of Chongqing Medical University,Chongqing,401331;Center for Mental Health,the First Affiliated Hospital of Chongqing Medical University,Chongqing,400016,China)

机构地区：[1]重庆医科大学附属大学城医院心理卫生中心,重庆401331 [2]重庆医科大学附属第一医院心理卫生中心,重庆400016

出　　处：《第三军医大学学报》2019年第11期1065-1069,共5页Journal of Third Military Medical University

基　　金：国家自然科学基金面上项目(81671360)~~

摘　　要：目的探讨脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)基因与神经营养性酪氨酸激酶受体2型(neurotrophic tyrosine kinase receptor type 2,NTRK2)基因交互作用与青年自杀未遂行为的关系。方法使用IMLDR^(tm)多重SNP分型技术对病例组(70例自杀未遂青年人)和对照组(112例健康青年人)的BDNF基因1个多态性位点rs1491851和NTRK2基因3个多态性位点rs1147198、rs11140800、rs1565445进行分型,采用广义多因子降维法(generalized multifactor dimensionality reduction,GMDR)分析基因-基因交互作用。结果在病例组与对照组中,BDNF rs1491851、NTRK2 rs1147198、rs11140800、rs1565445基因型频率与等位基因频率的分布差异均无统计学意义(P>0.05)。GMDR分析结果显示,BDNF基因与NTRK2基因的2～4阶交互作用,分别为两位点模型(rs1491851、rs1147198)、三位点模型(rs1491851、rs1147198、rs11140800)和四位点模型(rs1491851、rs1147198、rs11140800、rs1565445)。其中四位点模型(rs1491851、rs1147198、rs11140800、rs1565445,测试样本精度为0.632 5,交叉一致性检验为10/10,1 000次置换检验P值为0.032～0.033)为最佳模型。结论 BDNF基因与NTRK2基因存在交互作用,可能与青年自杀未遂行为相关。Objective To investigate the association of the interaction between brain-derived neurotrophic factor(BDNF) gene and neurotrophic tyrosine kinase receptor type 2(NTRK2) gene with suicide attempt in adolescents. Methods One single nucleotide polymorphism(SNP) of BDNF gene(rs1491851) and 3 SNPs of NTRK2(rs1147198, rs11140800, rs1565445) were tested by IMLDRtm multiplex SNP typing in 70 suicide attempters(15~24 years old) and 111 healthy young individuals(control). Analysis on gene-gene interaction was conducted by generalized multifactor dimensionality reduction(GMDR). Results No significant differences were found in genotype frequencies and allele frequencies between the attempters and controls at 4 SNPs(BDNF rs1491851, and NTRK2 rs1147198, rs1140800, rs1565445)(P>0.05). GMDR analysis indicated the interactions between BDNF gene and NTRK2 gene included a two-locus combination(rs1491851, rs1147198), a three-locus combination(rs1491851, rs1147198, rs11140800) and four-locus combination(rs1491851, rs1147198, rs11140800, rs1565445). The four-locus combination was identified as the best model(Bal. Acc. CV test: 0.632 5, CVC consistency: 10/10, permutation test: P=0.032~0.033). Conclusion There are interactions between BDNF gene and NTRK2 gene, which may be associated with suicide attempt in adolescents.

关 键 词：自杀未遂 脑源性神经营养因子 神经营养性酪氨酸激酶受体Ⅱ型 基因-基因交互作用 青年 

分 类 号：R181.23[医药卫生—流行病学] R394.1[医药卫生—公共卫生与预防医学]