2014-2017年中国部分地区PEDV流行株S基因遗传进化分析  

Phylogenetic analysis of S gene of porcine epidemic diarrhea virus from some districts of China during 2014-2017

作　　者：郑慧华 张鸿鑫 韩昊莹 乔涵[1] 赵宇 陈红英[1,2] ZHENG Huihua;ZHANG Hongxin;HAN Haoying;QIAO Han;ZHAO Yu;CHEN Hongying(College of Animal Science and Veterinary Medicine,Henan Agricultural University,Zhengzhou 450002;Zhengzhou Major Pig Disease Prevention and Control Laboratory,Henan Agricultural University,Zhengzhou 450002)

机构地区：[1]河南农业大学牧医工程学院,郑州450002 [2]郑州市猪重大疫病防控重点实验室,郑州450002

出　　处：《安徽农业大学学报》2019年第2期249-255,共7页Journal of Anhui Agricultural University

基　　金：河南省产学研合作计划项目(152107000003)资助

摘　　要：参考GenBank中PEDV经典CV777毒株基因组序列设计特异性引物,采用RT-PCR方法对临床采集的疑似PEDV样品进行S基因扩增、克隆和测序,拼接获得30条完整S基因序列,并进行遗传进化及同源性分析。结果显示,获得的30条PEDV S基因与25个参考株S基因的核苷酸相似性介于93.6%~99.8%;且各流行毒株与经典毒株比较,具有多处的点突变、插入和缺失。与其他参考株相比,S1区存在157个氨基酸突变位点,占总数的65.7%(157/239),暗示PEDV的S1区域比S2区域易发生变异;表明目前中国PEDV流行株已经发生了变异,在一定程度揭示了免疫猪群仍然发病的原因。To understand the genetic variation of the prevalent PEDV strains, specific primers were designed based on the genome sequence of the PEDV classical CV777 strain in GenBank. The S genes were amplified by RT-PCR from clinically suspected PEDV samples, and 30 complete sequences of S gene were obtained by sequencing and splicing. Further genetic evolution and homology analysis results showed S genes of the strains in this study had 93.6 to 99.8% nucleic acid sequence similarity to S gene of 25 reference strains, and each strain had multiple point mutations, insertions, and deletions. There were 157 amino acid mutations in S1 region, which accounted for 65.7%(157/239) of the total number of S gene mutation sites, indicating that the PEDV S1 region was more variable than the S2 region. Comparison of the deduced amino acid sequence of the S protein between each strain and the reference strain showed that the current PEDV epidemic strains have been mutated, and it has revealed to a certain extent the reason why the immune pig herd is still onset in China.

关 键 词：PEDV S基因 遗传进化分析 变异 

分 类 号：S858.28[农业科学—临床兽医学]