Gli1抑制剂GANT61对髓母细胞瘤细胞增殖和凋亡的影响  

Effects of Gli1 inhibitor GANT61 on proliferation and apoptosis of medulloblastoma cells

作　　者：卢恩敏 卢恩娟 高丽京 裴立静 王庆艳[1] 师翠云 LU Enmin;LU Enjuan;GAO Lijing;PEI Lijing;WANG Qingyan;SHI Cuiyun(People′s Hospital of Fengrun District, Tangshan 064000, China)

机构地区：[1]唐山市丰润区人民医院,河北唐山064000 [2]唐山市丰润区第二人民医院

出　　处：《山东医药》2019年第17期13-15,27,共4页Shandong Medical Journal

基　　金：河北省卫生厅科研基金项目(20181399)

摘　　要：目的 探讨Gli1抑制剂GANT61对髓母细胞瘤细胞增殖和凋亡的影响。方法 取髓母细胞瘤Daoy细胞,随机分为对照组和10、50、100μmol/LGANT61组,对照组加入0.1%DMSO,10、50、100μmol/LGANT61组分别加入10、50、100μmol/LGANT61和0.1%DMSO。采用MTT法测算细胞增殖抑制率,流式细胞仪检测细胞凋亡指数和细胞周期,qRT-PCR法检测细胞中Gli1、Bcl-2mRNA表达,Westernblotting法检测细胞中Gli1、Bcl-2蛋白表达。结果 各浓度GANT61组细胞增殖抑制率、细胞凋亡指数均高于对照组,且50、100μmol/LGANT61组高于10μmol/LGANT61组,100μmol/LGANT61组高于50μmol/LGANT61组(P均<0.05)。各浓度GANT61组G1期细胞比例均高于对照组、S期细胞比例均低于对照组,且50、100μmol/LGANT61组G1期细胞比例高于10μmol/LGANT61组、S期细胞比例低于10μmol/LGANT61组,100μmol/LGANT61组G1期细胞比例高于50μmol/LGANT61组、S期细胞比例低于50μmol/LGANT61组(P均<0.05)。各浓度GANT61组Gli1、Bcl-2mRNA和蛋白相对表达量均低于对照组,且50、100μmol/LGANT61组低于10μmol/LGANT61组,100μmol/LGANT61组低于50μmol/LGANT61组(P均<0.05)。结论 GANT61能够抑制髓母细胞瘤细胞的Gli1、Bcl-2mRNA及蛋白表达,从而抑制细胞增殖,增加G1期细胞比例,促进细胞凋亡。Objective To investigate the the effects of Gli1 inhibitor GANT61 on the proliferation and apoptosis of medulloblastoma cells. Methods The medulloblastoma Daoy cells were divided into four groups, the cells in the control group were added with 0.1% DMSO, and the cells in the other three experimental groups with 10, 50, 100 μmol/L GANT61;MTT was used to determine the cell proliferation activity, flow cytometry was used to detect the apoptosis and cell cycle. The qRT-PCR and Western blotting were used to detect the expression of Gli1 and Bcl-2. Results The cell proliferation inhibition rates and apoptosis index of the GANT61 groups (10, 50, and 100 μmol/L GANT61 groups) were higher than those of the control group, and with the increase of GANT61 concentration, the apoptosis index and inhibition rate increased, with statistically significant difference ( P <0.05). The number of cells in the G 1 phase of the GANT61 group with different levels was higher than that of the control group, with the increase of GANT61 levels, the proportion of cells in the G 1 phase increased, and the proportion of cells in the S phase decreased, with statistically significant difference (all P <0.05). The relative expression of Gli1 and Bcl-2 mRNA in the 10, 50, and 100 μmol/L GANT61 groups were lower than that of the control group (all P <0.05), and the expression level decreased with the increase of GANT61 levels, and statistically significant differences were found (all P <0.05). Conclusion GANT61 can inhibit the Gli1 and Bcl-2 mRNA and protein expression of medulloblastoma cells, thus inhibit the cell proliferation, increase the proportion of cells in the G 1 phase, and promote apoptosis.

关 键 词：髓母细胞瘤 GANT61 Gli1抑制剂 BCL-2基因 细胞增殖 细胞凋亡 

分 类 号：R739[医药卫生—肿瘤]