马铃薯萌芽出苗期根系对水分胁迫的响应机理分析  

Response of Roots of Potato at Seedling Stage to Water Stress

作　　者：王晓娇[1,2] 蒙美莲[1] 曹春梅[2] 逯春杏[2] 许飞 WANG Xiaojiao;MENG Meilian;CAO Chunmei;LU Chunxing;XU Fei(College of Agronomy, Inner Mongolia Agricultural University, Hohhot 010019, China;Inner Mongolia Academy of Agricultural & Animal husbandry Sciences, Hohhot 010031, China)

机构地区：[1]内蒙古农业大学农学院,呼和浩特010019 [2]内蒙古农牧业科学院,呼和浩特010031

出　　处：《灌溉排水学报》2019年第5期12-18,共7页Journal of Irrigation and Drainage

基　　金：国家现代农业产业技术体系建设专项(CARS-10-P17);内蒙古自治区农牧业科学院青年创新基金项目(2015QNJJN10);国家自然科学基金项目(31260416)

摘　　要：【目的】研究马铃薯根系对不同水分胁迫的响应机理。【方法】试验以马铃薯栽培品种‘克新1号’为材料,利用Illumina HiSeqTM 4000高通量测序技术,从转录组水平分析马铃薯萌芽出苗期根系对不同水分胁迫基因的差异表达,分析萌芽出苗期根系对水分胁迫应答的分子机理。【结果】①通过对转录组测序分析,中度水分胁迫样品中获得差异表达基因1 488个,其中上调578个,下调910个,重度水分胁迫样品中获得差异表达基因5 241个,其中上调1 905个,下调3 336个,共同差异表达基因369个,差异基因主要与蛋白激酶、水解酶、氧化还原酶、水通道蛋白、转运蛋白、转录因子等相关;②马铃薯萌芽出苗期根系中谷胱甘肽及其代谢途径参与了抵御水分胁迫处理的正调控;③激素ABA、ET和CKs信号转导被激活,IAA、属于12个家族的107个蛋白磷酸酶基因,可能参与水分胁迫应答,其中丝氨酸/苏氨酸磷酸酶(PP2C,PP2A)参与了ABA信号转导的调控;④发现了属于54个家族的488个转录因子基因,其中部分转录因子参与水分胁迫应答。【结论】在水分胁迫下,马铃薯萌芽出苗期根系基因出现差异表达,其中部分蛋白激酶、蛋白磷酸酶、氧化还原酶、转录因子及其参与的生物代谢途径和信号转导途径参与了水分胁迫的应答响应。【Objective】Root development at early stage has consequence for seasonal plant growth, and the purpose of this paper is to study the response of roots of potato at seedling stage to water stress.【Method】The variety of KEXIN 1 was used in the experiment. We analyzed the gene expression at transcriptional level using highthroughout sequencing of Illumina HiSeq TM 4000 from the transcriptome to study the response of root to water stress.【Result】① Expression of 1488 genes was identified at moderate water stress, in which 578 were up-regulated and 910 were down-regulated. At sever water stress, expression of 5 241 genes were identified, among which 1 905 were up-regulated and 3336 genes were down-regulated, with 369 regulated neither way. The difference in gene expression was associated with protein kinase, hydrolase, oxidoreductase, aquaporin, transporter,transcription factors, among others.②The glutathione and its metabolic pathway were involved in positive regulation to resist water stress during germination stage.③The signal transduction of hormones ABA, ET and CKs were activated, yet the transduction of IAA, JA and SA was inhibited. The signal transduction pathways of ABA and ET were involved in improving the resistance of plant to water stress.④ Under moderate to severe water stress, 409 protein kinase genes from 7 families and 107 protein phosphatase genes from 12 families were found to have expressed, which might contribute to the resistance of plant to water stress. Serine/threonine phosphatase(PP2 C, PP2 A) was involved in regulating the ABA signal transduction.⑤ There were 488 transcription genes from 54 families, some of which were involved in resistance of the plant to water stress.【Conclusion】Under water stress, genes in roots of potato seedling expressed differently. Some protein kinases, protein phosphatases, oxidoreductases, transcription and those involved in biological metabolic pathways and signal transduction pathways were involved in plant resistance to water stress.

关 键 词：马铃薯 水分胁迫 转录组 差异表达基因 响应机理 根系 

分 类 号：S532[农业科学—作物学]