Double Magnetic Separation-assisted Fluorescence Method for Sensitive Detection of Ochratoxin A  

作　　者：WANG Chengke TAN Rong LI Jiangyu ZHANG Zexiang 

机构地区：[1]College of Food and Biological Engineering,Jiangsu University,Zhenjiang 212013,P.R.China [2]Henan Key Laboratory of Biomolecular Recognition and Sensing,Shangqiu Normal University, Shangqiu 476000,P.R.China

出　　处：《Chemical Research in Chinese Universities》2019年第3期382-389,共8页高等学校化学研究（英文版）

基　　金：the National Natural Science Foundation of China(No.21305032);the China Postdoctoral Science Foundation (No.2014M551522);the Postdoctoral Science Foundation of Jiangsu Province, China(No.1402073B);the Henan Key Laboratory of Biomolecular Recognition and Sensing(Shangqiu Normal University), China(No.HKLBRSKl803);the Hong Kong Scholar Program, China (No. XJ2017008).

摘　　要：A double magnetic separation-assisted fluorescence method was developed to rapidly detect ochratoxin A(OTA). The OTA aptamer functionalized magnetic nanomaterial(Fe3O4-Aptanier) and complementary DNA conjugated nitrogen-doped graphene quantum dots(NGQDs-cDNA) were used in this assay. Aptamer could hybridize with cDNA, which induced tlie NGQDs-cDNA to bind onto Fe3O4-Aptamer, and resulted in the fluorescence quenching of NGQDs. After the addition of OTA, the NGQDs-cDNA could release into the solution, and resulted in the recovery of fluorescence signal of NGQDs consequently. By utilizing the magnetic separation, the unbonded NGQDs-cDNA and residual Fe3O4-Aptamer were removed, which significantly increased the fluorescence signal intensity. OTA could be detected in the linear range of 10 nmol/L to 2000 nmol/L, with a limit of detection as 0.66 mnol/L. The advantages of this method include simple operation, good selectivity and high sensitivity, and this method can be used for the rapid detection of ochratoxin A in wheat and com.

关 键 词：OCHRATOXIN A FE3O4 Magnetic SEPARATION FLUORESCENCE method APTAMER 

分 类 号：O6[理学—化学]