长期电针刺激对大鼠周围神经吻合术后感觉及运动功能恢复的影响  

Effect of Long-term Electroacupuncture Stimulation on the Recovery of Sensory and Motor Function after Peripheral Nerve Anastomosis in Rats

作　　者：胡春鹤[1] 金驰[2] 徐屹[1] 张晓[1] 张栋栋 杨庆健[1] HU Chun-he;JIN Chi;XU Yi(The third hospital of Hebei Medical University, Shijiazhuang,050000,China)

机构地区：[1]河北医科大学第三医院,河北石家庄050000 [2]河北省沧州市中心医院,河北沧州061000

出　　处：《医学临床研究》2019年第5期846-848,共3页Journal of Clinical Research

基　　金：河北省卫生厅科研项目(20180469).

摘　　要：【目的】通过建立周围神经损伤(PNI)大鼠模型评估长期电针刺激(EA)对PNI吻合术后感觉及运动功能恢复的影响。【方法】选择90只雄性大鼠,根据电脑随机数表法将其分为对照组、PNI组和PNI+EA组,每组各30只,PNI组和PNI+EA组大鼠通过横切坐骨神经建立PNI模型。PNI+EA组给予不连续波EA治疗,PNI组及对照组不作处理,试验共8周。应用腿部功能指数(LFI)、刺激反射阈值(ST)、温觉刺激阈值(TST)、神经传导功能(NCF)评估EA对PNI神经再生的影响。【结果】试验第1周时PNI + EA组、PNI组LFI值迅速下降,2~8周开始上升,5~7周PNI + EA组LFI值高于PNI组,但组间比较差异无统计学意义(P>0.05)。试验后2~8周,PNI + EA组、PNI组ST值逐渐下降,第8周时PNI + EA组显著低于PNI组,差异具有统计学意义(P<0.05)。第2~6周时PNI + EA组TST值逐渐降低,4~8周PNI + EA组显著低于PNI组,差异具有统计学意义(P<0.05)。试验8周时 PNI组、PNI + EA组NCF值低于对照组,PNI + EA组高于PNI组(P<0.05)。【结论】短期EA可能对大鼠损伤的周围神经再生作用不显著,长期持续刺激可促进神经再生和功能恢复。【Objective】To evaluate the effect of long-term electroacupuncture stimulation (EA) on sensory and motor function recovery after PNI anastomosis by the establishment of a rat model of peripheral nerve injury (PNI).【Methods】Ninety male rats were selected and divided into control group, PNI group and PNI+EA group according to the method of computer random number table. 30 rats in each group were divided into PNI group and PNI+EA group. The PNI model was established by transection of sciatic nerve in PNI group and PNI+EA group. The PNI + EA group was treated with discontinuous wave EA, while the PNI group and the control group were not treated. The experiment lasted for 8 weeks. The effects of EA on PNI nerve regeneration were assessed by leg function index (LFI), stimulus reflex threshold (ST), temperature stimulation threshold (TST) and nerve conduction function (NCF).【Results】The LFI values of the PNI + EA group and the PNI group decreased rapidly in the first week of the experiment, and began to rise in the second to the eighth week, while the LFI values of the PNI + EA group were higher than those of the PNI group in the fifth to seventh week, but there was no significant difference between the two groups (P>0.05). The ST value of the PNI + EA group and the PNI group decreased gradually from 2 to 8 weeks after the experiment. At the 8th week, the ST value of the PNI + EA group was significantly lower than that of the PNI group (P<0.05). The TST value of PNI + EA group decreased gradually at the 2nd to 6th week, and the TST value of the PNI + EA group was significantly lower than that of the PNI group at the 4th to 8th week (P<0.05). The NCF values of the PNI group and the PNI + EA group were lower than those of the control group at 8 weeks of experiment, while those of the PNI + EA group were higher than those of the PNI group (P<0.05).【Conclusion】Short-term EA may have no significant effect on the regeneration of injured peripheral nerves in rats. Long-term sustained stimulation can promote nerv

关 键 词：电针 大鼠 周围神经 吻合术 外科 

分 类 号：R745[医药卫生—神经病学与精神病学]