牙本质涎蛋白通过整合素β6调节Smad蛋白活性  

DSPaa183-219 Regulates Activity of Smad Protein by Interacting with Integrin β6

作　　者：勾晓辉 柴纪华 袁国华[1] GOU Xiao-hui;CHAI Ji-hua;YUAN Guo-hua(State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory for Oral Biomedicine of Ministry of Education (KLOBM),School and Hospital of Stomatology,Wuhan University,Wuhan 430079,China)

机构地区：[1]武汉大学口腔医学院湖北省口腔基础医学重点实验室——省部共建国家重点实验室培育基地口腔生物医学教育部重点实验室

出　　处：《口腔医学研究》2019年第6期537-540,共4页Journal of Oral Science Research

基　　金：国家自然科学基金(编号:81470708)

摘　　要：目的:研究牙本质涎蛋白片段DSP aa183-219 与成牙本质细胞膜上整合素β6结合后对细胞内Smad蛋白的影响。方法: DSP aa183-219 刺激后,免疫荧光和免疫印迹检测胞内Smad蛋白磷酸化和核转移情况。整合素β6抗体阻断β6受体,DSP aa183-219 刺激后检测胞内Smad蛋白磷酸化和核转移情况。结果: DSP aa183-219 刺激后,胞内磷酸化Smad1/5/8蛋白表达上调,并向核转移;整合素β6抗体阻断后,DSP aa183-219 刺激不再影响细胞内Smad1/5/8蛋白的磷酸化水平及向核转移情况。结论: DSP aa183-219 能够通过成牙本质细胞膜上整合素β6受体来提高胞浆内Smad1/5/8蛋白的磷酸化水平并促进磷酸化Smad1/5/8向核转移。Objective: To investigate the effects of DSP aa183-219 on the intracellular activity of Smad protein through binding to integrin β6. Methods: Immunofluorescence and western blot assay were used to detect the expression levels of Smad1/5/8 and phospho-Smad1/5/8 protein in the odontoblasts after treated with DSP aa183-219 fragments.Anti-β6 antibody was used to inhibit integrin β6,and immunofluorescence and western blot assay were used to determine the expression pattern of Smad1/5/8 protein in the odontoblasts after treated with DSP aa183-219 fragments. Results: After stimulated by DSP aa183-219 fragments,phosphorylated-Smad1/5/8 in the odontoblasts was up-regulated and translocated to the nucleus.After treated with integrin β6 inhibitor (anti-β6 antibody),and followed by adding DSP aa183-219 to the medium for different time periods,phosphorylation and nuclear translocation of Smad1/5/8 in the odontoblasts induced by DSP aa183-219 were effectively blocked. Conclusion: DSP aa183-219 binds to integrin β6 on the cell surface and induces Smad1/5/8 phosphorylation and nuclear translocation in the odontoblasts.

关 键 词：牙本质涎蛋白 整合素β6 SMAD蛋白 磷酸化 

分 类 号：R781[医药卫生—口腔医学]