Cytological Observation of Pollen Abortion of Lycium barbarum Haploids from Anther Culture  

作　　者：Qing LUO Bo ZHANG Guoli DAI Jianhua ZHAO Youlong CAO 

机构地区：[1]National Wolfberry Engineering Research Center

出　　处：《Medicinal Plant》2019年第2期21-24,共4页药用植物：英文版

基　　金：Supported by Natural Science Foundation of Ningxia Hui Autonomous Region(NZ16097);Special Agricultural Breeding Project of Ningxia Hui Autonomous Region(2013NYYZ0105,2018NYYZ01-03)

摘　　要：To reveal the sterility characteristics of Lycium barbarum haploids, cytological observations were made on the anthers of Ningqi No.1 and its haploids obtained from anther culture. The results showed that there were no significant differences in anther development between Ningqi No.1 and its haploids at the stage of pollen mother cell, and tetrads were formed successfully after the meiosis stage. The tetrads of Ningqi No.1 could release microspores. At the same time, the tapetal cells can provide nutrition for the development of the microspores, which eventually developed into mature pollen grains. Although the haploids could also release microspores at the tetrad stage, the tapetal cells degraded in advance, which made the released microspores unable to develop into mature pollen grains, resulting in pollen abortion of haploids.To reveal the sterility characteristics of Lycium barbarum haploids, cytological observations were made on the anthers of Ningqi No.1 and its haploids obtained from anther culture. The results showed that there were no significant differences in anther development between Ningqi No.1 and its haploids at the stage of pollen mother cell, and tetrads were formed successfully after the meiosis stage. The tetrads of Ningqi No.1 could release microspores. At the same time, the tapetal cells can provide nutrition for the development of the microspores, which eventually developed into mature pollen grains. Although the haploids could also release microspores at the tetrad stage, the tapetal cells degraded in advance, which made the released microspores unable to develop into mature pollen grains, resulting in pollen abortion of haploids.

关 键 词：LYCIUM barbarum ANTHERS HAPLOIDS POLLEN ABORTION Cell morphology 

分 类 号：R[医药卫生]