慢性根尖肉芽肿中TNF-α与IL-34表达的相关性  

Correlation between IL-34 and TNF-α in the chronic periapical granulomas

作　　者：朱永娜 陈直 宋伟[1] 张晓东[1] Yong-na Zhu;Zhi Chen;Wei Song;Xiao-dong Zhang(Department of Stomatology, the Second Affiliated Hospital of Bengbu Medical College, Bengbu,Anhui 233000, China;Department of Clinical Medicine, Bengbu Medical College,Bengbu, Anhui 233000, China)

机构地区：[1]蚌埠医学院第二附属医院口腔科

出　　处：《中国现代医学杂志》2019年第12期32-35,共4页China Journal of Modern Medicine

基　　金：蚌埠医学院自然科学基金(No:BYKY17119)

摘　　要：目的探讨慢性根尖肉芽肿中肿瘤坏死因子α(TNF-α)和白细胞介素-34(IL-34)表达的关系,并进一步研究TNF-α在慢性根尖肉芽肿中的致病机制。方法选取2018 年3 月—2018 年5 月就诊于蚌埠医学院第二附属医院且临床及影像学诊断为慢性根尖肉芽肿的患者。9 例根尖肉芽肿组织作为实验组。拔除智齿时切除的健康牙龈组织8 例作为对照组。应用免疫组织化学法检测两组TNF-α和IL-34 蛋白的表达,并应用图像处理软件分析两者的表达量及相关性。结果实验组TNF-α、IL-34 相对表达量高于对照组(P <0.05);实验组TNF-α与IL-34 呈正相关(r =0.896,P =0.001),对照组TNF-α与IL-34 无相关性(r =0.186,P =0.659)。结论在慢性根尖肉芽肿中TNF-α与IL-34 的表达有相关性。在慢性根尖肉芽肿中,TNF-α可能通过IL-34 发挥致病作用。Objective To study the correlation of TNF-α and IL-34 in the chronic apical granulomas and the pathogenic mechanism of TNF-alpha in chronic periapical granulomas. Methods Nine cases of chronic periapical granulomas were selceted as experimental samples. Eight cases of normal gingival tissues from the impacted third molars recommended for extraction were selected as normal control samples. The expressions of IL-34 protein and TNF-α protein in the experimental samples and control samples were detected by immunohistochemical analysis. The image J was used to analyze the expressions of IL-34 and TNF-α. Results The relative expression of TNF-a and IL- 34 in the experimental group was higher than that in the control group (P < 0.05);there was a positive correlation between TNF-a and IL-34 in the experimental group (r = 0.896, P = 0.001), but no correlation between TNF-a and IL-34 in the control group (r = 0.186, P = 0.659). Conclusions The relationship of TNF-a and IL-34 in the chronic periapical granulomas is demonstrated, and the TNF-α may play a pathogenic role through IL-34.

关 键 词：肉芽肿 牙根尖 慢性病 肿瘤坏死因子Α 

分 类 号：R781.34[医药卫生—口腔医学]