水通道蛋白3介导过氧化氢转运对宫颈癌HeLa细胞增殖、迁移的作用研究  

Inhibitory effect of aquaporin-3 on proliferation and migration of cervical carcinoma HeLa cells

作　　者：袁芳[1] 史永华[2] Fang Yuan;Yong-hua Shi(Basic Medical College of Xinjiang Medical University, Urumqi, Xinjiang 830011, China;Department of Pathology, Basic Medical College of Xinjiang Medical University,Urumqi, Xinjiang 830011, China)

机构地区：[1]新疆医科大学基础医学院,新疆乌鲁木齐830011 [2]新疆医科大学基础医学院病理教研室,新疆乌鲁木齐830011

出　　处：《中国现代医学杂志》2019年第11期19-24,共6页China Journal of Modern Medicine

基　　金：国家自然科学基金(No:81660427)

摘　　要：目的探讨水通道蛋白3(AQP3)介导肿瘤微环境中过氧化氢(H2O2)转运对宫颈癌He La细胞增殖、迁移的作用。方法应用宫颈癌He La细胞,构建AQP3 sh RNA慢病毒稳定转染宫颈癌细胞系,通过克隆形成、CCK-8、细胞平板克隆形成、Western blotting等比较H2O2处理前后细胞表型的差异。结果成功构建AQP3-464-sh RNA慢病毒稳定转染He La细胞系,AQP3 sh RNA抑制肿瘤微环境中H2O2转运,抑制宫颈癌He La细胞增殖、迁移,AQP3 shRNA抑制肿瘤微环境中H2O2转运调控下游信号蛋白激酶B(PKB)。结论 AQP3介导宫颈癌He La细胞中H2O2跨膜转运,激发细胞内依赖第二信使H2O2的下游信号PKB,促进He La细胞的增殖、迁移。Objective To investigate the role of aquaporin 3 (AQP3) in proliferation and migration of cervical cancer HeLa cells. Methods Construction of AQP3 shRNA overexpression in cervical cancer HeLa lines via lentivirus. CCK-8, cell clone formation and Western blotting were performed for phenotype conformation. Results AQP3-464-shRNA overexpression in HeLa cell line via lentivirus was successfully constructed. AQP3 shRNA inhibited hydrogen peroxide transportation in tumor microenvironment. Inhibition of proliferation and migration of cervical cancer HeLa cells was achieved through AQP3 shRNA. Treatment of AQP3 shRNA inhibited AKT activation. Conclusions AQP3 promotes the proliferation and migration of HeLa cells through transportation of H2O2 and downstream AKT signaling pathway.

关 键 词：宫颈肿瘤 过氧化氢 水通道蛋白3 细胞增殖 迁移 

分 类 号：R737.33[医药卫生—肿瘤]