三七发酵液多糖抗衰老活性研究  

Study on anti-aging activity of polysaccharides in Sanqi fermentation liquor

作　　者：刘平平[1] 虞旦[1] 王昌涛[1,2] 杨帆 李萌[1] 张佳婵[1] LIU Ping-ping;YU Dan;WANG Chang-tao;YANG Fan;LI Meng;ZHANG Jia-chan(School of Science, Beijing Technology and Business University, Beijing 100048, China;Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, China;Yunnan Baiyao Group Co., Ltd., Kunming, Yunnan 650501, China)

机构地区：[1]北京工商大学理学院北京市植物资源研究开发重点实验室,北京100048 [2]北京工商大学北京食品营养与人类健康高精尖创新中心,北京100048 [3]云南白药集团股份有限公司,云南昆明650501

出　　处：《日用化学工业》2019年第6期369-376,387,共9页China Surfactant Detergent & Cosmetics

基　　金：北京工商大学两科基金培育项目资助(LKJJ2016-9);北京市教委科技计划面上项目资助(KM201310011007);微生物技术升级枸杞副产物资源化利用与示范资助项目(2016-SF-121)

摘　　要：采用食品级黄酒酵母发酵三七,得三七发酵液后对其进行分离纯化得到三七多糖。通过生化水平以及细胞水平的实验评价三七发酵液多糖的抗衰老功效。结果显示:三七发酵液多糖对DPPH自由基、羟自由基、超氧阴离子自由基的半抑制浓度IC50分别为1.528,0.798和0.216g/L,发酵三七多糖可以显著降低胞内活性氧水平;显著降低成纤维细胞培养液中MMP-1含量;显著提高细胞I型胶原蛋白含量,在发酵三七多糖对成纤维细胞的安全质量浓度范围内,检测10个与衰老相关的因子的表达量变化,只有col-Iα(I型胶原α)表达量下调,其他因子表达量均上调,其中促血管形成因子Ang-1表达量最高。以上结果说明三七发酵液多糖具有一定的抗衰老功效。The fermentation liquor of Sanqi(Panax notoginseng)was obtained by the fermentation of Sanqi with yellow rice wine yeast. Sanqi polysaccharides were separated from such liquor and then purified. The anti-aging effect of polysaccharides in fermentation liquor of Sanqi was evaluated both on biochemical level and cellular level. The results show that, the semi-inhibitory concentrations(IC50)against DPPH free radical, hydroxyl radical and superoxide anion free radical are 1.528, 0.798 and 0.216 g/L, respectively. The polysaccharides from the fermentation liquor of Sanqi can significantly reduce both the levels of intracellular reactive oxygen and the content of MMP-1 in fibroblast culture medium, and they can also significantly increase the content of cell type I collagen. In the safe mass concentration range of fermented sanqi polysaccharides on fibroblasts, 10 senescencerelated factors were detected, only col-Iα(type I collagen)is down-regulated, and all other factors are upregulated, among which the expression of angiogenic factor Ang-1 is the highest. The above results indicate that the polysaccharides from Sanqi fermentation liquor has certain anti-aging effect.

关 键 词：黄酒酵母 三七多糖 自由基 抗衰老 化妆品 

分 类 号：TQ658[化学工程—精细化工]