植物乳杆菌CGMCC8198破碎上清液对黑色素生成的抑制作用  

Inhibitory Effect of Lactobacillus plantarum CGMCC8198 Lysis Supernatant on Melanogenesis

作　　者：张彩姣 俞晓亭 潘丽娟 周浩[1] 张同存[1] 罗学刚[1] ZHANG Caijiao;YU Xiaoting;PAN Lijuan;ZHOU Hao;ZHANG Tongcun;LUO Xuegang(Key Lab of Industrial Fermentation Microbiology,Ministry of Education,Tianjin Key Lab of Industrial Microbiology,College of Biotechnology,Tianjin University of Science & Technology,Tianjin 300457,China)

机构地区：[1]工业发酵微生物教育部重点实验室天津市工业微生物重点实验室天津科技大学生物工程学院

出　　处：《天津科技大学学报》2019年第3期15-21,共7页Journal of Tianjin University of Science & Technology

基　　金：国家重点研发计划资助项目(2017YFD0400303);国家高技术研究发展计划(863计划)资助项目(2012AA022108)

摘　　要：黑色素是由黑色素细胞合成的一种生物色素,能避免皮肤被紫外线灼伤,过度增长或分布不均会影响美观,导致老年斑、雀斑、黑斑病等病症.通过对植物乳杆菌(Lactobacillus plantarum)CGMCC8198破碎上清液(LAS)的抗氧化和黑色素生成抑制活性进行检测,结果表明:LAS能明显清除ABTS和DPPH自由基,并显著降低小鼠黑色素瘤细胞B16F10内黑色素的生成,且对细胞活性几乎无影响.LAS可降低细胞内的黑色素生成关键酶——酪氨酸酶的活性,而对细胞外酪氨酸酶的活性则无影响.通过实时荧光定量PCR(Real-timePCR)、免疫印迹实验(Westernblot)和荧光素酶报告基因检测分析,结果表明:LAS能够抑制酪氨酸酶和其相关蛋白家族成员TRP-1、TRP-2及该家族上游关键转录调控因子小眼畸形相关转录因子(MITF)的转录表达;抑制MITF以及TYR启动子转录活性.Melanin is a biological pigment synthesized by melanocytes,which can prevent the skin from being burned by UV rays. Overgrowth or uneven distribution of melanin can affect the appearance and lead to senile plaques,freckles,black spots and other diseases. The antioxidization ability and melanogenesis inhibition of the cell lysis supernatant(LAS)prepared from Lactobacillus plantarum CGMCC8198 were tested. LAS can clearly scavenge free radicals ABTS and DPPH. In B16F10 melanoma cells,LAS significantly reduced the intracellular melanin production,but did not affect the cell activity. Then,the effects of LAS on the activity of tyrosine and the key enzyme of melanin production were analyzed. LAS reduced the activity of intracellular tyrosinase and had no effect on the activity of extracellular tyrosinase. The effect of LAS was analyzed by RT-PCR,Western blot and Luciferase reporter assay. LAS could inhibit not only the transcription and expression of tyrosinase,tyrosinase-related protein TRP-1 and TRP-2,but also their key regulation factor MITF. The results of luciferase reporter assay show that LAS could inhibit the promoter activity of both TYR and MITF.

关 键 词：植物乳杆菌 黑色素 抗氧化 MITF 

分 类 号：TQ658[化学工程—精细化工]