基于适冷酶研究的生物综合实验教学设计  

Teaching Design of Comprehensive Biology Experiment Based on Cold-adapted Enzymes

作　　者：王全富 侯艳华 王一帆 王亚彤 WANG Quanfu;HOU Yanhua;WANG Yifan;WANG Yatong(School of Marine Science and Technology,Weihai Campus of Harbin Institute of Technology,Weihai 264209,Shandong,China)

机构地区：[1]哈尔滨工业大学威海校区海洋科学与技术学院

出　　处：《实验室研究与探索》2019年第6期149-151,161,共4页Research and Exploration In Laboratory

基　　金：国家自然科学基金项目(41876149,31100037);山东省自然科学基金面上项目(ZR2017MC046);哈尔滨工业大学创新实验课建设项目(IP07000110)

摘　　要：通过引入专业热点问题,并结合学校海洋培养特色,生物综合实验教学设计以海洋微生物适冷酶为材料,对其基因的克隆与生物信息学进行分析。采用同源建模分析手段,分析适冷酶的结构特点;利用亲和层析方法对重组适冷酶进行分离纯化;SDS-PAGE分析基因工程产物及纯度鉴定,研究重组蛋白活性。通过综合实验使学生深入了解海洋微生物适冷酶基因,挖掘学生的创新潜力,提升学生的实验操作技能,进而达到提高实验教学质量的目的。Through the introduction of scientific issues,and in combination with the marine training features of Harbin Institute of Technology,the genes of cold-adapted enzymes from marine microorganisms were cloned and the bioinformatics were analyzed in the comprehensive biology experiment. Then the structure characteristics of cold-adapted enzymes were also analyzed using homology modeling. The recombinant enzyme was purified using affinity chromatography. Besides,the genetic engineering products and identify the purity were analyzed by SDS-PAGE. The recombinant enzyme activities were also determined. The comprehensive biology experiment could make students a deep understanding of the research on the genes of cold-adapted enzymes from marine microorganisms,improved their potential for innovation and experimental operation skills,and then achieved the purpose of improving the quality of experiments.

关 键 词：生物综合性实验 适冷酶 教学研究 

分 类 号：G642.0[文化科学—高等教育学]