模板中CGC^+数量对纳米银铂簇性能影响的探讨  

Influence of CGC^+ Number in Triplex-DNA Template on Properties of Silver-platinum Bimetallic Nanoclusters

作　　者：傅微微 林倩 肖瑞煊 陈敬华[1] 吴芳[1] FU Weiwei;LIN Qian;XIAO Ruixuan;CHEN Jinghua;WU Fang(School of pharmacy,Fujian Medical University,Fuzhou,Fujian 350122,China)

机构地区：[1]福建医科大学药学院

出　　处：《闽南师范大学学报（自然科学版）》2019年第2期66-71,共6页Journal of Minnan Normal University：Natural Science

基　　金：国家自然科学基金(21874019);福建省自然科学基金(2019J01681)

摘　　要：对四种含不同数量胞嘧啶-鸟嘌呤-胞嘧啶三连体(CGC^+)的银铂双金属纳米簇的结构及催化活性进行研究,探讨模板中CGC^+数量对其性能的影响.方法以含不同数量CGC^+的DNA三倍体为模板合成四种银铂纳米簇,采用透射电镜、紫外可见分光光度、荧光光谱等方法,研究模板中CGC^+数量对纳米簇结构及催化活性的影响.结果表明,模板中CGC^+数量可影响纳米簇结构,四种簇均有类过氧化物酶活性,能催化H2O2氧化TMB,且活性随CGC^+数量增加而有所增强.该研究可为纳米簇的合成及应用提供新思路和参考.The structure and catalytic activity of four kinds of triplex silver-platinum nanoclusters (triplex-AgPtNCs) containing different amounts of CGC^+ were studied, and the effect of CGC^+ number on the properties was discussed. Templated by the triplex DNA, four kinds of triplex-AgPtNCs were synthesized, respectively. Then the structure and catalytic activity of these triplex-AgPtNCs were studied by ultraviolet-visible spectrophotometry, fluorescence spectrometry and transmission electron microscope. Results showed the four kinds of triplex silver platinum nanoparticles had peroxidase-like activity, which can catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine by H2O2. Moreover, the catalytic activity had been enhanced along with the increase of the number of CGC^+. Meanwhile, it was also found that the number of CGC^+ in template sequences would affect the uniformity of nanoclusters. The study may provide new train of thought or reference for the synthesis and application of nanoclusters.

关 键 词：DNA三倍体 银铂纳米簇 CGC^+数量 荧光光谱 类过氧化物酶活性 

分 类 号：O643[理学—物理化学]