HER2在乙肝肝硬化患者血清中的表达及临床意义  

Expression and clinical significance of HER2 in serum of patients with hepatitis B cirrhosis

作　　者：宋凯晴 仓慧 潘虹 高昳 沈卫东[1] SONG Kaiqing;CANG Hui;PAN Hong;GAO Yi;SHEN Weidong(Department of Gastroenterology, the Affiliated Jiangyin Hospital of Nantong University, Jiangsu 214400)

机构地区：[1]南通大学附属江阴医院消化内科

出　　处：《交通医学》2019年第3期225-227,234,共4页Medical Journal of Communications

摘　　要：目的:检测乙肝后肝硬化患者血清人类表皮生长因子受体2(HER2)浓度,并探讨其临床意义。方法:采集江阴市人民医院60例慢性乙肝、149例乙肝后肝硬化患者及60例健康体检人群血清标本,采用时间分辨荧光免疫分析法检测血清HER2水平,比较不同组间、不同肝硬化严重程度患者间血清HER2水平,分析乙肝肝硬化患者血清HER2水平与临床参数及终末期肝病模型(MELD)评分的相关性。结果:血清HER2浓度在健康对照组、慢性乙肝组、乙肝后肝硬化组分别为9.21±2.59 ng/mL、11.59±4.43 ng/mL和17.60±6.03 ng/mL,与健康对照组相比,慢性乙肝、乙肝后肝硬化患者血清HER2水平均显著升高(P<0.01),乙肝肝硬化组表达水平显著高于慢性乙型肝炎组(P<0.001),差异均有统计学意义。肝硬化Child C级患者血清HER2(16.57±4.67 ng/mL)高于Child A级(12.11±3.65 ng/mL,P=0.000)及B级(14.48±4.53 ng/mL,P=0.02)患者,差异均有统计学意义。失代偿期肝硬化患者HER2水平与BIL、AST、ALT、PT呈正相关(P均<0.05),与Alb水平呈负相关(P<0.05),但与MELD评分无相关性。结论:乙肝肝硬化患者血清HER2水平高表达,且与其严重程度呈正相关,提示HER2可能参与乙肝后肝硬化的进程。Objective: To detect the serum human epidermal growth factor receptor 2 (HER2) concentration in patients with post-hepatitis B cirrhosis and to explore its clinical significance. Methods: Serum samples were collected from 60 patients with chronic hepatitis B, 149 patients with post-hepatitis B cirrhosis and 60 healthy people in Jiangyin People's Hospital. Serum HER2 levels were detected by time-resolved fluorescence immunoassay(TRFIA) and were compared among different groups and patients with different severity of liver cirrhosis. The correlations between serum HER2 level and clinical parameters and end-stage liver disease model (MELD) score in patients with hepatitis B cirrhosis were analyzed. Results: The serum HER2 levels in the healthy control group, the chronic hepatitis B group and the post-hepatitis B cirrhosis group were 9.21±2.59 ng/mL, 11.59 ±4.43 ng/mL, and 17.60±6.03 ng/mL, respectively. Compared with the healthy control group, the serum HER2 levels in the chronic hepatitis B and post-hepatitis B cirrhosis patients were significantly higher (P<0.01), and the expression level in the hepatitis B cirrhosis group was significantly higher than that in the chronic hepatitis B group (P<0.001), and all the differences were statistically significant. The serum HER2 (16.57±4.67 ng/mL) in the patients with cirrhosis at Child C level was higher than those at Child A level (12.11±3.65 ng/mL, P=0.000) and B level (14.48±4.53 ng/mL, P=0.02). HER2 level was positively correlated with BIL, AST, ALT and PT in decompensated cirrhosis patients (P<0.05), negatively correlated with Alb level (P<0.05), but not with MELD score. Conclusion: Serum level of HER2 is high in patients with hepatitis B cirrhosis, and is positively correlated with the severity of hepatitis B cirrhosis, suggesting that HER2 may be involved in the process of post-hepatitis B cirrhosis.

关 键 词：人类表皮生长因子受体2 乙肝肝硬化:时间分辨荧光免疫分析法 

分 类 号：R575.2[医药卫生—消化系统]