干扰素α刺激角质形成细胞表达HLA-I/II和CD40分子的研究  

Interferon alpha stimulates expression of keratinocytes HLA-I/II and CD40 molecules

作　　者：宋晨成 王宇峰[1] 孙倩倩[1] 魏巍 唐国瑶[1] SONG Chen-cheng;WANG Yu-feng;SUN Qian-qian;WEI Wei;TANG Guo-yao(Department of Oral Mucosa, The Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, National Center for Clinical Research of Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai Institute of Stomatology, Shanghai 230001, China)

机构地区：[1]上海交通大学医学院附属第九人民医院口腔黏膜科国家口腔疾病临床医学研究中心上海市口腔医学重点实验室上海市口腔医学研究所,上海230001

出　　处：《临床口腔医学杂志》2019年第5期259-263,共5页Journal of Clinical Stomatology

基　　金：国家自然科学基金(81570975;81400512);中华口腔医学会专项基金(CSA-Z2015-03);国家临床重点专科建设项目资助(国卫办医函[2013]554)

摘　　要：目的:探究干扰素(interferon,IFN)α刺激角质形成细胞后,角质形成细胞表面共刺激分子CD40、CD80、CD86和提呈相关分子HLA-I、HLA-DR的变化以及IFN-α对角质形成细胞生存曲线的影响。方法:以人永生化口腔黏膜角质形成细胞(human immortlized oral mucosal keratinocytes,HOK)和Ha Ca T细胞为细胞模型,以IFN-α(实验组)、IFN-γ(阳性对照组)、PBS(阴性对照组)分别刺激细胞,以流式细胞术检测细胞表面CD40、CD80、CD86、HLA-I、HLA-DR的变化。结果:与阴性对照组相比,IFN-α实验组的角质形成细胞在刺激后24 h,HLA-I类分子、HLA-DR和CD40的阳性细胞比例及平均荧光强度明显提高。结论:IFN-α可刺激角质形成细胞表达HLA-I类分子、HLA-DR和CD40分子。Objective: To investigate the effects of IFN-α on the expression of co-stimulatory molecules CD40,CD80,CD86 and related molecules HLA-I and HLA-DR after IFN-α stimulation of keratinocytes and the survival curve of keratinocytes in IFN-α.Methods: Human immortalized oral mucosal keratinocytes and Ha Ca T cells were used as cell models,and IFN-α( experimental group),IFN-γ( positive control group),and PBS( negative control group) were used to stimulate cells to flow cells.The changes of CD40,CD80,CD86,HLA-I and HLA-DR on the cell surface were detected.Results: Compared with the negative control group,the proportion of HLA-I molecules,HLA-DR and CD40 positive cells and the average fluorescence intensity of keratinocytes in the IFN-α experimental group were significantly increased 24 hours after stimulation.Conclusion: IFN-α can stimulate keratinocytes to express HLA-I molecules,HLA-DR and CD40 molecules.

关 键 词：扁平苔藓 角质形成细胞 干扰素Α HLA分子 CD40 

分 类 号：R781.5[医药卫生—口腔医学] Q813.5[医药卫生—临床医学]