移植微囊化嗅鞘细胞对神经病理性疼痛及L_(4-5)段脊髓P2X7受体表达的影响  

Effects of microencapsulated olfactory sheathing cells transplantation on neuropathic pain and the expression of P2X7 receptor in L_(4-5) spinal cord

作　　者：张文俊 熊梅娟[1] 周七保 孙弋 刘曾旭[2] ZHANG Wen-jun;XIONG Mei-juan;ZHOU Qi-bao;SUN Yi;LIU Zeng-xu(Department of Anatomy, Basic Medical School, Jinggangshan University, Jiangxi Ji'an 343000, China;Department of Anatomy, Basic Medical School, Nartchang University, Nanchang 330000, China)

机构地区：[1]井冈山大学基础医学院解剖学教研室,江西吉安343000 [2]南昌大学基础医学院解剖学教研室,南昌330000

出　　处：《解剖学报》2019年第3期280-284,共5页Acta Anatomica Sinica

摘　　要：目的探讨微囊化嗅鞘细胞移植对坐骨神经损伤引起病理性疼痛及L_(4-5)段脊髓P2X7受体的表达影响。方法取1只健康SD大鼠,取其嗅球组织并运用差速贴壁方法,在体外进行嗅鞘细胞培养扩增。取48只健康SD大鼠,随机分成4组,即对照组(control)、慢性压迫性坐骨神经损伤组(CCI)、嗅鞘细胞移植组(OECs)及微囊化嗅鞘细胞移植组(MC-OECs)。术后7 d及14 d,运用行为学方法检测各组大鼠机械缩足反射阈值,运用原位杂交方法检测各组大鼠L_(4-5)段脊髓P2X7受体阳性细胞百分比及平均吸光度表达情况。结果术后7 d及14 d,与对照组比较,CCI组大鼠机械缩足反射阈值明显减低,L_(4-5)段脊髓P2X7受体阳性细胞百分比及平均吸光度明显增加(P<0.05);与CCI组比,OECs组大鼠机械缩足反射阈值明显增高,P2X7受体阳性细胞百分比及平均吸光度明显减低(P<0.05),与OECs组对比,MC-OECs组大鼠机械缩足反射更高。P2X7受体阳性细胞百分比及平均吸光度更低(P<0.05)。结论微囊化嗅鞘细胞移植能更好地缓解神经病理性疼痛,减低L_(4-5)段脊髓内P2X7受体的表达水平,修复坐骨神经损伤。Objective To investigate the effect of microencapsulated olfactory sheathing cells transplantation on pathological pain induced by peripheral nerve injury and the expression levels of P2 X7 receptor in L4-5 spinal cord. Methods Nash differential adherence method was used to cultivate and expand cells from olfactory bulb tissue of a SD rat. Forty healthy SD rats were randomly divided into control group, model group, OECs group and microencapsulated OECs group(MC-OECs groups). At the 7 th and 14 th days after surgery, the mechanical stimulation constraining thresholds of the rats in each group were measured by behavioral method. The experssion levels of P2 X7 receptor positive cells percentage and average absorbance by in situ hybridization were observed in L4-5 spinal cord. Results At the 7 th and 14 th days after surgery, compared with the control group, the reflex threshold of mechanical withdrawl of rats in the CCI group significantly decreased(P<0.05). The percentage and average absorbance of P2 X7 receptor positive cells in L4-5 spinal cord significantly increased(P<0.05)compared with the CCI group, the reflex threshold of mechanical withdrawl of rats in the OECs group was significantly increased(P<0.05). The percentage and average absorbance of P2 X7 receptor positive cells in L4-5 spinal cord significantly decreased compared with the OECs group(P<0.05). The reflex threshold of mechanical withdrawl of rats in the MC-OECs group was higher(P<0.05). The percentage and average absorbance of P2 X7 receptor positive cells in L4-5 spinal cord were lower(P<0.05). Conclusion Microencapsulated olfactory ensheathing cells transplantation can relieve the neuropathic pain better and reduce the expression levels of P2 X7 receptor and repair peripheral nerve injury than OECs transplantate.

关 键 词：嗅鞘细胞 微囊化 移植 L4.5段脊髓 P2X7受体 原位杂交 大鼠 

分 类 号：R322[医药卫生—人体解剖和组织胚胎学]