咸味觉厌恶模型大鼠鼓索神经对味觉刺激的电生理反应特性  

The electrophysiological response of chorda tympani nerve to taste stimuli in rats with conditioned taste aversion to saltiness

作　　者：李腊梅[1,2] 廖莹莹 蒋恩社 LI La-mei;LIAO Ying-ying;JIANG En-she(Institute of Nursing and Health,Henan University,Kaifeng 475004;Department of Nursing,Zhengzhou Central Hospital Affiliated toZhengzhou University,Zhengzhou 450007,China)

机构地区：[1]河南大学护理与健康研究所,开封475004 [2]郑州大学附属郑州中心医院,郑州450007

出　　处：《中国应用生理学杂志》2019年第3期239-244,共6页Chinese Journal of Applied Physiology

基　　金：河南省高等学校重点科研项目(19A180013)

摘　　要：目的:探索大鼠咸味觉厌恶建立后外周鼓索神经(CT)对咸味觉及其他味觉刺激的电生理反应特性的改变。方法:将14只SD成年雄性大鼠分为咸味觉厌恶模型组(CTA)和对照组(n=7/group)。实验第1日给予大鼠30min的0.1mol/LNaCl饮食,随后CTA组和对照组大鼠分别腹腔注射2ml0.15mol/LLiCl和同等量生理盐水。在第2、3和4日,测量两组大鼠每天30min内对NaCl和蒸馏水饮用量。于第4日行为学测试后,分别记录CTA组大鼠和对照组大鼠CT对口内给予系列浓度NaCl溶液、0.3mol/LNaCl与0.1mmol/L阿米洛利(一种舌上皮钠通道阻断剂)混合液和其他四种基本味觉刺激溶液的电生理反应。结果:与对照组相比,CTA组大鼠CT对系列浓度NaCl和其他4种基本味觉刺激的电生理反应特性没有发生明显变化(P>0.05);舌上皮钠通道阻断剂阿米洛利强烈抑制CTA大鼠对NaCl的反应(P<0.01)。结论:条件性咸味觉厌恶模型大鼠CT对各种味觉刺激的电生理反应特性没有发生明显改变。Objective: To explore the characteristic changes of the peripheral chorda tympanic nerve( CT) electrophysiological responses to salty stimulus and other taste stimuli in rats with the conditioned taste aversion to saltiness. Methods: Fourteen adult SD male rats were divided into a conditioned taste aversion to salty group( CTA) and a control group( Ctrl)( n = 7/group). On the first day of the experiment,rats were given a 0.1 mol/L Na Cl intake for 30 min,then,the rats in CTA and Ctrl groups were injected intraperitoneally with 2 ml of 0.15 mol/L Li Cl and the same amount of saline respectively. On day 2,3 and 4,the 30 min consumption of Na Cl and distilled water was measured for both groups of rats. On the 4 th day after the behavioral test of that day,CT electrophysiological recording experiments were performed on CTA rats and control rats. Results: Compared with the rats in Ctrl group,the electrophysiological characteristics of CT in CTA group rats did not change significantly the responses to the series of Na Cl and other four basic taste stimuli( P>0.05). The amiloride,the epithelial sodium channel blocker,strongly inhibited the response of CT to Na Cl in CTA and Ctrl group rats( P<0.01). Conclusion: The electrophysiological responses of CT to various gustatory stimuli do not significantly change in rats after the establishment of conditional taste aversion to the saltiness.

关 键 词：条件性味觉厌恶 鼓索神经 味觉 大鼠 

分 类 号：R338[医药卫生—人体生理学]