高效液相色谱法测定鲜虾中4-己基间苯二酚残留量的不确定度分析  

Uncertainty evaluation for the determination of 4-Hexylresorcinol residue in shrimp by high performance liquid chromatography

作　　者：芦江会 王小宝 张书芬 应璐 陈爱媚 傅晓 周子焱 LU Jiang-hui;WANG Xiao-bao;ZHANG Shu-fen;YING Lu;CHEN Ai-mei;FU Xiao;ZHOU Zi-yan(Ningbo Institute for Food Control,Ningbo 315048)

机构地区：[1]宁波市食品检验检测研究院

出　　处：《中国食品添加剂》2019年第6期128-134,共7页China Food Additives

摘　　要：采用高效液相色谱法对鲜虾中4-己基间苯二酚残留量测定的不确定度进行评估。根据JJF1135-2005《化学分析测量不确定度评定》和JJF1059.1-2012《测量不确定度评定与表示》规定的测量不确定度的基本方法,建立数学模型,分析鲜虾中4-己基间苯二酚残留量测定的不确定度的来源,并对各不确定度分量进行量化和合成,得到当鲜虾中的检测结果为1.01mg/kg时,其扩展不确定度为0.094mg/kg(k=2)。通过评定比较各影响分量可知,影响检测结果的不确定度的主要因素为待测物浓度和回收率,重复性测定次之,样品称量、试样定容引入的不确定度较小。Evaluation uncertainty for the determination of 4-hexylresorcinol residue in shrimp by high performance liquid chromatography was carried out.According to the basic method of uncertainty measurement from JJF1135-2005(Evaluation of Uncertainty in Chemical Analysis of Measurement)and JJF1059.1-2012(Evaluation and Expression of Uncertainty),a mathematical model was established to evaluate the uncertainty of determining the 4-hexylresorcinol residues in shrimp.The possible uncertainty sources were analyzed and each component of uncertainty was quantified and combined.The result showed that the expended uncertainty was 1.01 mg/kg with 0.094mg/kg(k=2)residues.Through the evaluation and comparison of the influence components,it can be seen that the main factors affecting the uncertainty of test results are the concentration and recovery rate of the substance to be tested,followed by repeatability measurement,and the uncertainty introduced by sample weighing and sample constant volume have less influences.

关 键 词：高效液相色谱法 鲜虾 4-己基间苯二酚 不确定度 

分 类 号：O657.7[理学—分析化学] TS207.5[理学—化学]