Long-term Fiber Photometry for Neuroscience Studies  

作　　者：Yi Li Zhixiang Liu Qingchun Guo Minmin Luo 

机构地区：[1]National Institute of Biological Sciences,Beijing 102206,China [2]Thinker Tech Nanjing Biotech Limited Co.,Nanjing 210019,China [3]School of Life Sciences,Tsinghua University,Beijing 100084,China

出　　处：《Neuroscience Bulletin》2019年第3期425-433,共9页神经科学通报（英文版）

基　　金：the Ministry of Science and Technology of China(2012YQ03026005,2013ZX0950910,and2015BAI08B02);the National Natural Science Foundation of China(91432114,91632302);the Beijing Municipal Government

摘　　要：Fiber photometry is a sensitive and easy way to detect changes in fluorescent signals. The combination of fiber photometry with various fluorescent biomarkers has substantially advanced neuroscience research over the last decade. Despite the wide use of fiber photometry in biomedical fields, the lack of a detailed and comprehensive protocol has limited progress and sometimes complicated the interpretation of data. Here, we describe detailed procedures of fiber photometry for the long-term monitoring of neuronal activity in freely-behaving animals, including surgery, apparatus setup, data collection, and analysis.Fiber photometry is a sensitive and easy way to detect changes in ?uorescent signals. The combination of?ber photometry with various ?uorescent biomarkers has substantially advanced neuroscience research over the last decade. Despite the wide use of ?ber photometry in biomedical ?elds, the lack of a detailed and comprehensive protocol has limited progress and sometimes complicated the interpretation of data. Here, we describe detailed procedures of ?ber photometry for the long-term monitoring of neuronal activity in freely-behaving animals,including surgery, apparatus setup, data collection, and analysis.

关 键 词：Ca^(2+) signal Fluorescence detection Ca^(2+) indicator Optical fiber Experiment protocol 

分 类 号：R[医药卫生]