Effects and mechanism of total phenols of Magnolia officinalis combined with Maijunan Tablets on blood pressure of spontaneous hypertensive rats  

作　　者：Ming-li Zhu Wei Wang Feng Qin Yong Chen 

机构地区：[1]Key Laboratory of Biotechnology of Hubei Province, Key Laboratory of Biotechnology of Chinese Traditional Medicine, National & Local Joint Engineering Research Center of High-throughput Drug Screening Technology, Hubei University

出　　处：《Chinese Herbal Medicines》2019年第2期177-184,共8页中草药（英文版）

基　　金：supported by the major Technological innovation project of Hubei Province (Grant No. 2016ACA140);the united fund for innovation and entrepreneurship of Ministry of Education of China (Grant No. 201610512001)

摘　　要：Objective: Maijunan(MJA) Tablets is a protected variety of traditional Chinese medicine(TCM) consisted of Pueraria lobata, hydrochlorothiazide(HTCZ), Uncaria rhynchophylla(366:1:980) and excipient. In the present work, MJA was consisted of the total flavones of P. lobata, HCTZ and total alkaloids of U. rhynchophylla(40:11:75). The combination of MJA and the total phenols of Magnolia officinalis(M-MJA) was consisted of the total flavones of P. lobata, the total phenols of M. officinalis, HCTZ and the total alkaloids of U. rhynchophylla(40:40:11:75). The aim of this work was to examine the effect and mechanism of M-MJA on the blood pressure of spontaneous hypertensive rats(SHRs).Methods: Adult male SHRs were randomly divided into control group, MJA group(180 mg/kg·d), and the M-MJA group(218 mg/kg·d)(n = 5). SHRs were orally administered with M-MJA and MJA respectively once a day for 8 weeks, the blood pressure of SHRs was measured every two weeks, and the biochemical indicators related to blood pressures were detected at the last dosing.Results: After oral administration of M-MJA to SHRs once a day for 8 weeks, the systolic and diastolic blood pressures of SHRs were deceased significantly. M-MJA affected renin-angiotensin-aldosterone system by decreasing the levels of Ren, Ang II and ALD, affected the endothelial function by decreasing the levels of ET-1 and 20-HETE, and increasing the level of eNOS, affected the oxidative stress by increasing the protein expression of Nrf2 and the activities of HO-1 and GSH-Px, and decreasing the protein expression of CYP2 E1 and CYP4 A, as well as the content of MDA.Conclusion: These results indicated that M-MJA could regulate the renin-angiotensin-aldosterone system,improve endothelial function, and inhibit CYP4 A activity to reduce the production of 20-HETE, alleviate the oxidative stress disorder of the visceral organs, and eventually exert antihypertensive effect. Additionally, the anti-oxidant ability, regulating the renin-angiotensin-aldosterone system and improving endoObjective: Maijunan(MJA) Tablets is a protected variety of traditional Chinese medicine(TCM) consisted of Pueraria lobata, hydrochlorothiazide(HTCZ), Uncaria rhynchophylla(366:1:980) and excipient. In the present work, MJA was consisted of the total flavones of P. lobata, HCTZ and total alkaloids of U. rhynchophylla(40:11:75). The combination of MJA and the total phenols of Magnolia officinalis(M-MJA) was consisted of the total flavones of P. lobata, the total phenols of M. officinalis, HCTZ and the total alkaloids of U. rhynchophylla(40:40:11:75). The aim of this work was to examine the effect and mechanism of M-MJA on the blood pressure of spontaneous hypertensive rats(SHRs).Methods: Adult male SHRs were randomly divided into control group, MJA group(180 mg/kg·d), and the M-MJA group(218 mg/kg·d)(n = 5). SHRs were orally administered with M-MJA and MJA respectively once a day for 8 weeks, the blood pressure of SHRs was measured every two weeks, and the biochemical indicators related to blood pressures were detected at the last dosing.Results: After oral administration of M-MJA to SHRs once a day for 8 weeks, the systolic and diastolic blood pressures of SHRs were deceased significantly. M-MJA affected renin-angiotensin-aldosterone system by decreasing the levels of Ren, Ang II and ALD, affected the endothelial function by decreasing the levels of ET-1 and 20-HETE, and increasing the level of eNOS, affected the oxidative stress by increasing the protein expression of Nrf2 and the activities of HO-1 and GSH-Px, and decreasing the protein expression of CYP2 E1 and CYP4 A, as well as the content of MDA.Conclusion: These results indicated that M-MJA could regulate the renin-angiotensin-aldosterone system,improve endothelial function, and inhibit CYP4 A activity to reduce the production of 20-HETE, alleviate the oxidative stress disorder of the visceral organs, and eventually exert antihypertensive effect. Additionally, the anti-oxidant ability, regulating the renin-angiotensin-aldosterone system and improving endo

关 键 词：endothelial function Maijunan Tablets oxidative stress renin-angiotensin-aldosterone system spontaneously HYPERTENSIVE rats total PHENOLS of MAGNOLIA OFFICINALIS 

分 类 号：R[医药卫生]