微波超声协同萃取法提取不同芦笋废弃物中黄酮类物质的研究  

Extration on the flavonoid from different kinds of asparagus waste by ultrasonic-microwave synergistic extraction

作　　者：孟晓萌[1] 郑晓冬[1] 潘少香[1] 闫新焕[1] 刘雪梅[1] 谭梦男 李志成[1] 宋烨[1] MENG Xiaomeng;ZHENG Xiaodong;PAN Shaoxiang;YAN Xinhuan;LIU Xuemei;TAN Mengnan;LI Zhicheng;SONG Ye(Jinan Fruit Research Institute,All China Federation of Supply & Marketing Co-operatives,Jinan 250014)

机构地区：[1]中华全国供销合作总社济南果品研究院

出　　处：《食品科技》2019年第5期208-212,共5页Food Science and Technology

基　　金：“十三五”国家重点研发计划项目(2017YFF0207800)

摘　　要：基于微波超声协同萃取方式,提取经热风烘干和真空冷冻干燥处理的白芦笋废弃物及绿芦笋废弃物中的黄酮类物质,并对其提取率进行了分析比较。实验优化了微波超声萃取的最佳工艺,确定了最佳提取溶剂为80%的乙醇,微波功率为250W,提取时间为150s,料液比为1:30,超声频率为40Hz。样品中黄酮提取率的大小分别为:真空冷冻干燥处理绿芦笋废弃物(0.391%)>热风烘干处理绿芦笋废弃物(0.366%)>真空冷冻干燥处理白芦笋废弃物(0.014%)>热风烘干处理白芦笋废弃物(0.0088%)。该方法与传统方法相比,具有快速、高效、经济的优势,更适用于大批量样品中黄酮的提取,推进了芦笋废弃物资源的开发和综合利用。In this paper, the ultrasonic-microwave synergistic extraction was used to extract flavonoid from white asparagus waste and green asparagus. The samples were prepared by hot air drying and vacuum freeze-drying technique respectively. Their extration rate were compared. The optimum experiment condition was determined as follows: 80% ethanol was selected as extraction solution, 250 W was the microwave power, 150 s was the extraction time, 1:30 was the solid-to-liquid ratio and 40 Hz was ultrasonic frequency. The extraction rate of flavonoid was showed in sequence: vacuum freeze-drying green asparagus waste(0.391%)>hot air drying green asparagus waste(0.366%)>vacuum freeze-drying white asparagus waste(0.014%)>hot air drying green asparagus waste(0.0088%). This proposed method has the advantages of good speed, high efficiency and low cost compared with traditional method, which could be applied in large amount samples’ flavonoid extraction. Furthermore, this paper provided much useful information for the development and comprehensive utilization of asparagus waste.

关 键 词：芦笋废弃物 微波超声协同萃取 黄酮 真空冷冻干燥 

分 类 号：R284.2[医药卫生—中药学]