结直肠癌患者组织中CST1基因表达的相关研究及其临床意义  

Study on the expression of CST1 gene in colorectal cancer patients and its clinical value

作　　者：冯莉[1] 赵冬[1] 冯谢敏[2] 刘占军 王诗淼[1] FENG Li;ZHAO Dong;FENG Xie-min;LIU Zhan-jun;WANG Shi-miao(Department of Laboratory Affiliated Hospital of Yan'an University,Yanzan 716000,China;Department of Oncology,Affiliated Hospital of Yanan University,Yanzan 716000,China)

机构地区：[1]延安大学附属医院检验科,陕西延安716000 [2]延安大学附属医院肿瘤科,陕西延安716000

出　　处：《中国中西医结合消化杂志》2019年第6期441-446,共6页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

基　　金：延安市科学技术研究发展计划项目(No:2015HM-06-01)

摘　　要：[目的]探讨CST1在结直肠癌中的表达及其临床意义。[方法]采用实时荧光定量PCR(quantitative real-time polymerase chain reaction,qRT-PCR)检测课题组2013^2015年收集的195例确诊结直肠癌患者的癌组织及对应癌旁正常组织中CST1的表达量,并根据其表达量高低分为高表达组和低表达组;分析CST1表达量与结直肠癌患者临床特征、生存期之间的关系;通过过表达CST1的表达后检测其对结直肠癌细胞系增殖、迁移和侵袭能力的影响。[结果]CST1在结直肠癌组织中表达量高于对应癌旁正常组织(0.0062±0.0198vs.0.0032±0.0139,P=0.026);CST1高表达与结直肠癌患者分期(χ^2=16.465,P<0.001)、是否原位癌(χ^2=10.739,P=0.013)、淋巴结转移(χ^2=14.461,P=0.001)、远端转移(χ^2=17.278,P<0.001)相关;CST1高表达与结直肠癌患者生存期明显缩短(χ^2=13.04,P<0.001)CST1过表达后促进结直肠癌细胞增殖(F=43.88,P=0.0002)、迁移(78.0±6.7 vs.51.2±10.6,P<0.001)和侵袭(63.6±8.0vs.43.0±9.3,P<0.001)相关。[结论]CST1在结直肠癌中高表达,且高表达将缩短结直肠癌患者生存期,过表达CST1能够促进结直肠癌细胞增殖、迁移和侵袭能力。[Objective]To detect the expression pattern and clinic value of CST1 in colorectal cancer.[Methods]Use quantitative real-time polymerase chain reaction to detect the expression level of CST1 in 195 paired colorectal cancer tissues and corresponding normal tissues which were collected from 2013 to 2015,Expression levels equal to or higher than the median were defined as high,while levels lower than the median were defined as low.Analyze the relationship between the clinic characteristics of the patients and the expression levels,and relationship between the overall survival time of the patients and the expression levels.Detect the role of CST1 in cell proliferation,migration and invasion of colorectal cancer cells through ectopic overexpression of CST1.[Results]CST1 was upregulated in colorectal cancer(0.0062±0.0198 vs.0.0032±0.0139,P=0.026),the high expression level of CST1 was correlated with the clinical stage of patients(χ^2=16.465,P<0.001),T stage(χ^2=10.739,P=0.013),N stage(χ^2=14.461,P=0.001)and M stage(χ^2=17.278,P<0.001);colorectal cancer patients with upregulated CST1 had a shorter overall survival time(χ^2=13.04,P<0.001),overexpression of CST1 could promote cell proliferation(F=43.88,P=0.0002),migration(78.0±6.7 vs.51.2±10.6,P<0.001)and invasion(63.6±8.0 vs.43.0±9.3,P<0.001).[Conclusion]CST1 was overexpressed in colorectal cancer,overexpression of CST1 demonstrated a shorter overall survival rate in colorectal cancer patients.

关 键 词：结直肠癌 CST1 预后 

分 类 号：R735.3[医药卫生—肿瘤]