年龄因素对牙周膜干细胞多向分化能力的影响  

The effect of aging on the multi-directional differentiation ability of human periodontal ligament stem cells

作　　者：李晓钰 王红 谷秀格 魏福兰[1] LI Xiaoyu;WANG Hong;GU Xiuge;WEI Fulan(Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Department of Orthodontics, School of Stomatology, Shandong University, Jinan 250012, China)

机构地区：[1]山东大学口腔医学院口腔组织再生重点实验室山东大学口腔医院正畸科

出　　处：《口腔生物医学》2019年第2期78-82,共5页Oral Biomedicine

基　　金：国家自然科学基金(81771030,81470709);泰山青年基金(tsqn20161068)

摘　　要：目的:探讨年龄因素对人牙周膜干细胞(PDLSCs)多向分化能力的影响。方法:选取因阻生需要拔除的具有完整牙根的第三磨牙共14颗,根据患者年龄分为青少年组(16~19岁)和中老年组(38~50岁),分别刮取牙周膜组织,分离培养获得PDLSCs,用流式细胞术分析干细胞表面标记物;通过体外成骨、成脂诱导培养,比较两组PDLSCs的多向分化能力。结果:经过流式细胞术检测,两组PDLSCs均具有间充质干细胞的特性,但中老年组PDLSCs的成骨、成脂分化能力较青少年组明显减弱(P<0.05)。结论:PDLSCs的多向分化能力随年龄的增加而降低。Objective: To investigate the effect of aging on the multi-directional differentiation ability of human periodontal ligament stem cells (PDLSCs). Methods: 14 healthy third molars were collected with complete roots in clinic and divided into two groups according to the donors’ ages: the younger group (donor age at 16-19 years) and the older group (donor age at 38-50 years). The periodontal ligament tissues were obtained and PDLSCs were isolated and cultured. Flow cytometry analysis was used to identify the phenotypes of PDLSCs. To compare the multi-directional differentiation ability of PDLSCs, cells were subjected to osteogenic and adipogenic induction in vitro respectively. Results: Flow cytometry analysis revealed that PDLSCs in both groups had the characteristics of mesenchymal stem cells. And the multi-directional differentiation ability of PDLSCs in the older group was significantly decreased compared with that in the younger group( P <0.05). Conclusions: The multi-directional differentiation ability of PDLSCs decreased with age.

关 键 词：牙周膜干细胞 年龄 分化能力 

分 类 号：R780.2[医药卫生—口腔医学]