川芎嗪通过PTEN/AKT通路抑制外阴鳞癌SW962细胞增殖、侵袭和转移  

Tetramethylpyrazine(TMP) inhibiting proliferation, invasion and metastasis of vulvar squamous cell carcinoma SW962 cells through PTEN/AKT pathway

作　　者：杨劭劼 刘畅 卢烨 董思雨 王威[1] 李东阳 刘雨琪 武昕[1] Yang Shaojie;Liu Chang;Lu Ye;Dong Siyu;Wang Wei;Li Dongyang;Liu Yuqi;Wu Xin(Department of Gynecology,the First Affiliated Hospital of China Medical University,Liaoning Shenyang 110001,China;Department of Gynecology and Obstetrical,The 4th People's Hospital of Shenyang,Liaoning Shenyang 110031,China;Central Laboratory,Jinqiu Hospital of Liaoning Province,Liaoning Shenyang 110016,China;Department of Obstetrical,The First Affiliated Hospital of China Medical University,Liaoning Shenyang 110001,China)

机构地区：[1]中国医科大学附属第一医院妇科,辽宁沈阳110001 [2]沈阳市第四人民医院妇产科,辽宁沈阳110031 [3]辽宁省金秋医院中心实验室,辽宁沈阳110016 [4]中国医科大学附属第一医院产科,辽宁沈阳110001

出　　处：《现代肿瘤医学》2019年第14期2430-2435,共6页Journal of Modern Oncology

基　　金：国家自然科学基金(编号:30973190);辽宁省科学技术计划项目(编号:2014021057)

摘　　要：目的:研究和探讨川芎嗪对人外阴鳞癌细胞SW962细胞功能(增殖、凋亡、转移、侵袭)的影响以及机制。方法:将不同浓度川芎嗪配置而成的培养基干预处理外阴鳞癌SW962细胞。MTT法检测川芎嗪梯度浓度变化和处理时间变化对SW962细胞增殖和生存率的影响;流式细胞术检测川芎嗪梯度浓度变化对SW962细胞凋亡的影响;Transwell小室实验检测川芎嗪梯度浓度变化对SW962细胞转移和侵袭的影响;划痕实验检测川芎嗪梯度浓度变化对SW962细胞转移的影响;Western blot电泳法检测川芎嗪对PTEN、AKT、p-AKT、cyclinD1蛋白表达的影响。结果:川芎嗪显著抑制SW962细胞增殖(P<0.05),诱导SW962细胞凋亡增加细胞凋亡率(P<0.05),抑制SW962细胞转移和侵袭(P<0.05)。Western blot结果显示,川芎嗪处理细胞可以增加抑癌基因PTEN的表达,降低p-AKT和cyclinD1蛋白的表达(P<0.05)。结论:川芎嗪可以通过影响PTEN/AKT/cyclinD1信号通路抑制外阴鳞癌细胞SW962的增殖,诱导凋亡,抑制SW962细胞转移和侵袭。Objective:To study and investigate the effects and mechanisms of Tetramethylpyrazine(TMP) treated on the cell function(proliferation,apoptosis,metastasis,invasion) of human vulvar squamous cell carcinoma SW962 cells.Methods:Different concentrations culture medium of TMP was used to treat the vulvar squamous cell carcinoma SW962 cells.The effects of gradient concentration and treatment-time on the proliferation and survival rate of SW962 cells were detected by MTT assay.The effects of gradient concentration of TMP on apoptosis of SW962 cells was detected by flow cytometry.The effects of gradient concentration of TMP on metastasis and invasion of SW962 cells was detected by Transwell chamber test and scratch test.Western blot analysis to detect the protein changes of PTEN,AKT,p-AKT and cyclinD1 expression of TMP.Results:TMP significantly inhibited the proliferation of SW962 cells(P<0.05),induced apoptosis of SW962 cells(P<0.05),increased apoptosis-rate,and inhibited SW962 cell metastasis and invasion(P<0.05).Western blot analysis showed that TMP treatment increased the expression of PTEN and decreased the expression of p-AKT and cyclinD1 protein(P<0.05).Conclusion:TMP can inhibit the proliferation of vulvar squamous cell carcinoma cell line SW962 by inducing PTEN/AKT/cyclinD1 signaling pathway,induce apoptosis and inhibit metastasis and invasion of SW962 cells.

关 键 词：外阴鳞癌 SW962 PTEN 增殖 凋亡 侵袭 转移 

分 类 号：R737.35[医药卫生—肿瘤]