Glycosylation on proteins of the intestine and perimicrovillar membrane of Triatoma (Meccus) pallidipennis, under different feeding conditions  

作　　者：Ana E.Gutierrez-Cabrera Wesley F.Zandberg Edgar Zenteno Mario H.Rodriguez Bertha Espinoza Carl Lowenberger 

机构地区：[1]CONACyT-Centro de Investigacion Sobre Enfermedades Infecciosas,Instituto Nacional de Salud Publica,Cuernavaca,Morelos,Mexico [2]Department of Chemistry,Simon Fraser University,Burnaby,BC,Canada [3]Department of Chemistry,The University of British Columbia, Okanagan,Kelowna,BC,Canada [4]Facultad de Medicina,Departamento de Bioquimica,Universidad Nacional Autdmoma de Mexico, Mexico City,DF,Mexico [5]Centro de Investigacion Sobre Enfermedades Infecciosas,Instituto Nacional de Salud Publica,Cuernavaca, Morelos,Mexico [6]Departamento de Inmunologia,Instituto de Investigaciones Biomedicas,Universidad Nacional Autonoma de Mexico, Ciudad Universitaria,Mexico City,DF,Mexico [7]Department of Biological Sciences,Simon Fraser University,Burnaby,BC,Canada

出　　处：《Insect Science》2019年第5期796-808,共13页昆虫科学（英文版）

摘　　要：Trypanosoma cruzi,the causative agent of Chagas disease, interacts with molecules in the midgut of its insect vector to multiply and reach the infective stage. Many studies suggest that the parasite binds to midgut-specific glycans. We identified several glycoproteins expressed in the intestine and perimicrovillar membrane (PMM) of Triatoma (Meccus) pallidipennis under different feeding conditions. In order to assess changes in protein-linked glycans, we performed lectin and immunoblot analyses on glycoprotein extracts from these intestinal tissues using well-characterized lectins, and an antibody, which collectively recognize a wide range of different glycans epitopes. We observed that the amount and composition of proteins and glycoproteins associated with different glycans structures changed over time in the intestines and PMM under different physiological conditions. PMM extracts contained a wide variety of glycoproteins with different sugar residues, including abundant high-mannose and complex sialylated glycans. We propose that these molecules could be involved in the process of parasite-vector interactions.

关 键 词：feeding conditions GLYCOPROTEINS LECTINS perimicrovillar MEMBRANE TRIATOMINE vector-parasite interactions 

分 类 号：Q[生物学]