比较3种磷酸铵盐溶液制备碳酸磷灰石材料的理化性能  

Physical and chemical properties of carbonate apatite monolith treated by three ammonium phosphate solutions

作　　者：林欣[1] 侯敏[2] Lin Xin;Hou Min(Department of Prothodontics,Stomatological Hospital, Tianjin 300041,China;Department of Orthographic Surgery, Tianjin Stomatological Hospital,Tianjin 300041,China)

机构地区：[1]天津市口腔医院口腔修复科,天津市300041 [2]天津市口腔医院整形外科,天津市300041

出　　处：《中国组织工程研究》2019年第26期4136-4141,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:课题组前期研究通过氢氧化钙的碳酸化处理制备了方解石团块,并在低温磷酸二氢铵盐溶液中处理合成了碳酸磷灰石团块,其具有良好的理化性能,但存在碳酸含量偏低等不足,考虑与磷酸二氢铵盐溶液较低的pH值有关。目的:比较不同pH值磷酸铵盐溶液处理对方解石形成碳酸磷灰石骨替代材料理化性能的影响,寻找出在低温下制备出具有优良性能碳酸磷灰石团块的最理想磷酸铵盐溶液。方法:将团块状氢氧化钙进行碳酸化处理生成方解石,分3组处理,分别浸泡于磷酸二氢铵盐、磷酸氢二铵盐、磷酸三铵盐溶液中(浓度均为1 mol/L,温度均为60℃)。浸泡1,4,7,14 d后,分别进行径向抗拉强度测试、X射线衍射分析、傅里叶变换红外线分析、扫描电镜观察及碳酸含量、钙磷比等理化性能检测。结果与结论:(1)X射线衍射、傅里叶变换红外线、扫描电镜、径向抗拉强度结果显示:经过3种磷酸铵盐溶液处理14d后,CaCO3均已完全转化成为碳酸磷灰石,经NH4H2PO4水溶液处理的反应最快,最终样本达到最高的机械强度(10MPa),但碳酸含量相对最低(4.80±0.5)%;经(NH4)2HPO4与(NH4)3PO4水溶液处理的最终样本径向抗拉强度值均约为6MPa,可满足低负重部位骨缺损重建材料的机械强度要求;(2)化学分析结果显示:(NH4)3PO4水溶液处理最终样本的碳酸含量最高(7.25±0.81)%,与骨极其接近。并且B型碳酸磷灰石含量最高;(3)结果显示:3种磷酸铵盐溶液均可合成具有足够强度的低结晶性碳酸磷灰石团块,比较之下经(NH4)3PO4水溶液进行处理的最终产物最为理想。BACKGROUND: In our previous study, we prepared calcite monolith by carbonation of Ca(OH)2 and then synthesized dahllite agglomerates with encouraging physical and chemical properties after treatment with NH4 H2 PO4 solution at low temperature. But it had low carbonic acid content, which is considered to be associated with the low pH values of ammonium dihydrogen phosphate solution.OBJECTIVE: To compare the effects of ammonium phosphate solutions at different pH values on the physical and chemical properties of the carbonate apatite bone substitute material prepared by calcite and find the optimal ammoniumm phosphate solution for fabrication of carbonate apatite monolith with excellent performance at low temperature.METHODS: The agglomerated calcium hydroxide was carbonated to form calcite. The prepared calcite was soaked in three 1 mol/L ammonium phosphate solutions(NH4 H2 PO4,(NH4)2 HPO4,(NH4)3 PO4) at 60°C respectively. After 1, 4, 7 and 14 days of soaking, diametral tensile strength determination, X-ray diffraction analysis, Fourier transform infrared spectroscopy studies, and scanning electron microscopy were performed. The results were analyzed by comparison.RESULTS AND CONCLUSION: X-ray diffraction analysis, Fourier transform infrared spectroscopy, scanning electron microscopy, and diametral tensile strength determination results showed that after 14 days of treatment with three ammonium phosphate solutions, CaCO3 had completely transformed into carbonate apatite. The reaction rate was fastest in the NH4 H2 PO4 solution, with the highest diametral tensile strength of 10 MPa, but relative lower carbonate content(4.80±0.5)%. The diametral tensile strength of final product was 6 MPa after treatment by both(NH4)2 HPO4 and(NH4)3 PO4 solution. It is adequate for bone substitute used in the low-bearing parts. Chemical analyses showed that the carbonate content of the product treated by(NH4)3 PO4 solution was(7.25±0.81)%, which was extremely similar to that of bone. In addition, there was a high amount of B-type

关 键 词：碳酸磷灰石 磷酸铵盐溶液 人工骨 径向抗张强度 B型碳酸磷灰石 生物吸收性 pH值 碳酸含量 

分 类 号：R459.9[医药卫生—治疗学] R318.08[医药卫生—临床医学]