芸薹根肿菌侵染早期甘蓝型油菜转录组分析  

Transcriptome analysis of Brassica napus-Plasmodiophora brassicae interaction during early infection

作　　者：罗延青 王云月[1] 俎峰[2] 符明联[2] 赵凯琴[2] 张云云[2] 王敬乔[2] 田正书[2] 陈苇 李劲峰[2] 原小燕[2] 李根泽[2] LUO Yan-qing;WANG Yun-yue;ZU Feng;FU Ming-lian;ZHAO Kai-qin;ZHANG Yun-yun;WANG Jing-qiao;TIAN Zheng-shu;CHEN Wei;LI Jin-feng;YUAN Xiao-yan;LI Gen-ze(College of Plant Protection,Yunnan Agricultural University, Kunming Yunnan 650201,China;ndustrial Crops Research Institute, Yunnan Academy of Agricultural Sciences, Kunming Yunnan 650205, China)

机构地区：[1]云南农业大学植物保护学院,云南昆明650201 [2]云南省农业科学院经济作物研究所,云南昆明650205

出　　处：《中国油料作物学报》2019年第3期421-434,共14页Chinese Journal of Oil Crop Sciences

基　　金：云南省创新团队(2017HC021);云南省现代农业油菜产业技术体系建设(2017KGTX005);云岭产业技术领军人才;国家现代农业油菜产业技术体系(NYCYTX-00564);云南省创新人材培养对象项目(2017HB086)

摘　　要：根肿病是油菜最为严重的病害之一。为探索根肿病菌和油菜互作中的抗性分子机制,采用RNA-seq技术对根肿病早期侵染中抗病品系ZHE-226(R)和感病油菜10159(S)进行转录组分析。与感病材料S相比,在0、12、48和72h 4个接种时间点,抗性材料R中共有809个基因上调表达,1082个基因下调表达。模式识别受体、几丁质酶、R基因、WRKY、水杨酸和茉莉酸等抗性相关(PRR)基因在抗病材料R和感病材料S中诱导表达并表现出不同的表达模式。抗病材料R中PRR相关基因主要表现为下调表达,R基因上调表达,水杨酸表现为前期下调后期上调表达,茉莉酸、几丁质酶和WRKY因子在R和S中均诱导表达。结果表明PRR介导的PTI(病原相关分子模式触发的免疫反应)在感病材料S中显著诱导,在抗病材料R中作用不明显;R基因介导的ETI(效应蛋白触发的免疫反应)和水杨酸介导的抗病信号途径对根肿病抗性起重要作用。Clubroot is one of the most serious disease in oilseed rape.To explore the resistant molecular mechanism of the interaction between Plasmodiophora brassicae and Brassica napus during early infection,gene differential expression analysis of the clubroot-resistant rapeseed line ZHE-226(R) and clubroot-susceptible line10159(S) were performed by RNA-seq.A total of 809 and 1082 genes were commonly up-and down-regulated at 0,12,48,72 h post-inoculation(hpi) time points in R line.Pattern recognition receptors(PRRs),chitinase,R gene,WRKY transcription factor,salicylic acid(SA) and jasmonic acid(JA) were involved in the early response to clubroot infection in lines of R and S,but showed different expression patterns.Compared to S line,most of the PRRs genes were down-regulated,R genes were up-regulated and SA genes were down-regulated in earlier stage but up-regulated at 72 hpi in R line.Chitinase,WRKY and JA related genes were both induced in R and S lines.These results showed that PTI-mediated by PRRs was significantly induced in S line,but played a small role in R line.ETI-mediated by R gene and resistance signal pathway-mediated by SA played great role in clubroot resistance.This research provided a better understanding of the resistance genes of B.napus in response to P.brassicae,and assistance for clubroot-resistant breeding.

关 键 词：根肿病抗性 芸薹根肿菌 甘蓝型油菜 RNA-SEQ 

分 类 号：S565.403[农业科学—作物学]