磷酸化α-突触核蛋白在C57BL/6J小鼠边缘叶与大肠中的增龄性变化及相关性  

Correlative analysis of age-related changes of phosphorylated α-synuclein in limbic lobe and large intestine of C57BL/6J mice

作　　者：韩燕银 朱明慧 孔令东 骆降喜[1] 田顺亮[1] 陈敏[3] 刘承伟[3] 于兰[1] HAN Yan-Yin;ZHU Ming-Hui;KONG Ling-Dong(Guilin Medical University,Guilin 541004,Guangxi,China)

机构地区：[1]桂林医学院,广西桂林541004 [2]菏泽医学专科学校人体解剖学教研室 [3]桂林医学院附属医院

出　　处：《中国老年学杂志》2019年第13期3230-3233,共4页Chinese Journal of Gerontology

基　　金：国家自然科学基金地区基金(81760241);广西自然科学基金青年基金(2017GXNSFBA198019);广西重点研发计划(桂科AB18221039);广西脑与认知神经科学重点实验室开放课题(2017-6)

摘　　要：目的研究磷酸化α-突触核蛋白(P-α-syn)在C57BL/6J小鼠边缘叶与大肠中的增龄性变化及其相关性。方法蛋白质免疫印迹法(Western印迹)检测P-α-syn在不同年龄段C57BL/6J小鼠边缘叶与大肠中的表达变化,分析其相关性。结果P-α-syn表达量随小鼠年龄增加而增多,其中老年组与青年及中年组比较,差异具有统计学意义(P<0.01)。大肠中的P-α-syn水平与边缘叶P-α-syn的水平有相关性。结论C57BL/6J小鼠边缘叶与大肠中P-α-syn表达量随年龄的增加而增加,大脑边缘叶和大肠中P-α-syn表达变化呈正相关。Objective To study the age-related changes of phosphorylated α-synuclein (P-α-syn) in limbic lobe (LOB) and large intestine of C57BL/6J mice and their correlation. Methods The expression of P-α-syn in LOB and large intestine of C57BL/6J mice at different ages was detected by Western blot and its correlation was analyzed. Results The expression of P-α-syn increased with the age of the mice and there were significant differences between the aged mice and the young and middle-aged mice (P <0.01).The level of P-α-syn in the large intestine correlated with the level of LOB. Conclusions The expression of P-α-syn in LOB and large intestine of C57BL/6J mice increases with age.There is a positive correlation between the expression of P-α-syn in LOB and large intestine of mice.

关 键 词：Α-突触核蛋白 大肠 边缘叶 

分 类 号：R741[医药卫生—神经病学与精神病学]