LINC00158通过促进脾酪氨酸激酶基因的表达抑制膀胱癌细胞增殖和迁移  

Effects and mechanism of long-chain non-coding RNA LINC00158 on proliferation and migration of bladder cancer cells

作　　者：程树林[1] 李雨根[1] 陈双全[1] 黄静[1] 朱平宇[1] 龚志勇[1] CHENG Shulin;LI Yugen;CHEN Shuangquan;HUANG Jing;ZHU Pingyu;GONG Zhiyong.(Department of Urology,Affiliated Hospital of North Sichuan Medical College,Nanchong 637000,China)

机构地区：[1]川北医学院附属医院泌尿外科

出　　处：《实用医学杂志》2019年第11期1753-1756,1762,共5页The Journal of Practical Medicine

基　　金：四川省卫生和生育计划委员会基金(编号:18PJ456)

摘　　要：目的观察LINC00158对膀胱癌细胞功能的影响,并探究其可能的分子机制。方法荧光实时定量聚合酶链反应(qPCR)检测14例膀胱癌组织及癌旁组织、膀胱癌细胞株和正常膀胱上皮细胞中LINC00158的表达量。以表达量最低的膀胱癌细胞为转染对象,转染含有LINC00158全长的质粒(实验组)或转染对照质粒(对照组);qPCR检测转染后细胞中LINC00158和脾酪氨酸激酶(SYK)mRNA的表达;免疫印迹法(Western Blot)检测SYK蛋白的表达;细胞计数试剂盒(CCK-8法)和划痕实验分别检测LINC00158对膀胱癌细胞增殖和迁移能力的影响。结果 LINC00158在膀胱癌组织中的表达明显低于癌旁组织(P <0.01),在膀胱癌细胞株中的表达明显低于正常膀胱上皮细胞(P <0.05),在T24细胞中表达量最低(P <0.01)。LINC00158在实验组细胞中的表达明显高于对照组(P <0.01)。SYK在mRNA水平和蛋白水平的表达明显升高(P <0.05)。高表达LINC00158的细胞增殖能力明显降低(P <0.05),细胞迁移能力明显降低(P <0.01)。结论 LINC00158在膀胱癌组织和细胞中低表达,高表达LINC00158可以抑制膀胱癌细胞的增殖和迁移能力,其可能的分子机制是促进SYK基因的表达。Objective To observe the effect of LINC00158 on the function of bladder cancer cells and explore its possible molecular mechanisms. Methods Fluorescence real-time quantitative polymerase chain reaction(qPCR)was used to detect the expression of LINC00158 in 14 cases of bladder cancer tissues,paracancerous tissues,bladder cancer cell lines and normal bladder epithelial cells. The bladder cancer cells with the lowest expression rate were transfected with full-length plasmid LINC00158(experimental group)or transfected control plasmid(control group);qPCR was used to detect the expression of LINC00158 and spleen associated tyrosine kinase(SYK)mRNA;Expression of SYK protein was detected by Western Blot;Cell counting kit(CCK-8 method)and scratch test were used to detect the effect of LINC00158 on the proliferation and migration of bladder cancer cells. Results The expression of LINC00158 in bladder cancer tissues was significantly lower than that in adjacent tissues(P < 0.01),and the expression in bladder cancer cells was significantly lower than that in normal bladder epithelial cells(P < 0.05),and the expression in T24 cells was the lowest(P < 0.01). The expression of LINC00158 in the experimental group was significantly higher than that in the control group(P < 0.01). The mRNA and protein expression levels of SYK was significantly increased(P < 0.01). The proliferation of cells with high expression of LINC00158 was significantly decreased(P < 0.05),and the cell migration ability was significantly decreased(P < 0.01). Conclusions LINC00158 is lowly expressed in bladder cancer tissues and cells. High expression of LINC00158 can inhibit the proliferation and migration of bladder cancer cells. The possible molecular mechanism is to promote the expression of SYK gene,which provides an experimental basis for gene therapy of bladder cancer.

关 键 词：膀胱癌 长链非编码RNA 脾酪氨酸激酶 细胞增殖 细胞迁移 

分 类 号：R737.14[医药卫生—肿瘤]