CD19+CD24high CD38high调节性B细胞及相关细胞因子在视神经脊髓炎谱系疾病患者外周血中的变化及意义  

The study of CD19+CD24high CD38high regulatory B cells and related cytokines in the spectrum of optic nerve myelitis

作　　者：董梅[1] 史肖鹤 王炜[1] 李世平[1] 侯慧清[1] 吴冰洁[1] DONG Mei;SHI Xiaohe;WANG Wei(Department of Neurology,Key Laboratory of Neurology of Hebei Province,The Second Hospital of Hebei Medical University,Shijiazhuang 050000,China)

机构地区：[1]河北医科大学第二医院神经内科,神经病学河北省重点实验室,河北石家庄050000 [2]河北医科大学第三医院肌电图室,河北石家庄050000

出　　处：《中风与神经疾病杂志》2019年第6期496-500,共5页Journal of Apoplexy and Nervous Diseases

摘　　要：目的检测NMOSD患者外周血中CD19^+CD24^highCD38^high Breg及其细胞因子IL-10、TGF-β水平,探讨CD19^+CD24^highCD38^high Breg、IL-10、TGF-β在NMOSD急性期的变化及可能作用;检测NMOSD患者外周血GFAP水平,结合EDSS评分,探讨GFAP与疾病严重程度的相关性。方法选取2016年12月-2018年1月就诊于河北医科大学第二医院神经内科NMOSD急性期患者27例为实验组,性别、年龄相匹配的20例健康人为对照组。入组的NMOSD患者进行EDSS评分、流式细胞术检测外周血CD19^+CD24^highCD38^high Breg数量,PCR法检测外周血IL-10、TGF-β、GFAP的mRNA水平,并与健康人比较。结果NMOSD组CD19^+CD24^highCD38^high Breg水平较对照组下降,差异有统计学意义,CD19^+CD24^highCD38^high Breg与EDSS之间无线性关系。NMOSD组IL-10、TGF-β的mRNA水平较对照组下降,差异有统计学意义。NMOSD组GFAP水平较对照组升高,差异有统计学意义。GFAP与EDSS之间无线性关系。结论CD19^+CD24^highCD38^high Breg在NMOSD急性期发病中数量和功能上均受损,血清GFAP水平增高,但不能反应复发患者疾病残疾程度。Objective The levels of CD19^+CD24^highCD38^high Breg,IL-10 and TGF-beta in peripheral blood of patients with NMOSD were analyzed,and the role of CD19^+CD24^highCD38^high Breg in the acute phase of NMOSD was discussed.The level of GFAP in peripheral blood of NMOSD patients was detected and the correlation between GFAP and the severity of the disease was discussed with the EDSS score.Methods From December 2016 to 2018 01,27 patients in the acute phase of NMOSD in the Second Hospital of Hebei Medical University were selected as the experimental group,and 20 healthy persons matched for sex and age were selected as control group.The EDSS score and flow cytometry were used to detect the number of CD19^+CD24^highCD38^high Breg in peripheral blood of NMOSD patients.The levels of IL-10,TGF-and GFAP in peripheral blood were detected by PCR and compared with healthy persons.Results The level of CD19^+CD24^highCD38^high Breg in group of NMOSD was lower than that in the control group,and the difference was statistically significant.There was no linear relationship between Bregs and EDSS score.The levels of IL-10 and TGF-beta in group of NMOSD were lower than that in the control group,and the difference was statistically significant.The level of GFAP in group of NMOSD was higher than that in the control group,and there was a significant difference between the two groups.There is no linear relationship between GFAP and EDSS.Conclusion CD19^+CD24^highCD38^high Breg was not only significantly reduced in the pathogenesis of NMOSD,but also significantly impaired in its function.The serum GFAP increased but it has no relationship with EDSS in relapsed NMOSD patients.

关 键 词：视神经脊髓炎谱系疾病 白介素10 转化生长因子-β 胶质纤维酸性蛋白 

分 类 号：R774.6[医药卫生—眼科]