川楝素抑制裸鼠卵巢癌生长和促进肿瘤细胞凋亡  

Toosendanin inhibits the growth of ovarian cancer and promotes apoptosis in nude mice

作　　者：李少儒[1] 李燕[2] 户瑞丽[1] 文政芳[1] LI Shao-ru;LI Yan;HU Rui-li;WEN Zheng-fang(Department of Obstetrics and Gynecologythe First Affiliated Hospital of Xinxiang Medical College,Xinxiang 453100,China;Department of Endocrinology,the First Affiliated Hospital of Xinxiang Medical College,Xinxiang 453100,China)

机构地区：[1]新乡医学院第一附属医院妇产科,河南新乡453100 [2]新乡医学院第一附属医院内分泌科,河南新乡453100

出　　处：《基础医学与临床》2019年第7期1031-1035,共5页Basic and Clinical Medicine

摘　　要：目的观察川楝素对卵巢癌荷瘤裸小鼠肿瘤生长的抑制作用及其机制。方法将裸小鼠随机分组,移植卵巢癌细胞建模。阳性组腹腔注射环磷酰胺0.4 mL/d(10 g/L),实验1、2和3组分别取川楝素1、10和20 mg/kg溶于0.02 mL/g小鼠体质量0.9%氯化钠溶液中腹腔注射,1次/d,持续15 d。对比治疗后瘤体积、瘤质量和肿瘤组织HE染色结果,用TUNEL法检测细胞凋亡指数,以RT-PCR和Western blot检测P53、Bax、Bcl-2和caspase-9 mRNA及蛋白表达。结果模型组瘤体积和瘤质量均最高(P<0.05),细胞凋亡指数均最低(P<0.05);模型组肿瘤组织细胞密度大、排列紊乱、核深染、异型性明显;实验1、2、阳性组和实验3组细胞排列疏松、瘤细胞大小不一、核淡染、核仁小、瘤内可发现片状坏死和凋亡细胞;各组肿瘤组织Bax、Bcl-2和caspase-9 mRNA和蛋白表达量、p-P53/P53差异显著(P<0.05),实验2组和阳性组均相近,其余每2组间差异显著(P<0.05)。结论川楝素可抑制卵巢癌荷瘤裸鼠肿瘤生长,促进肿瘤细胞凋亡。Objective To observe the inhibitory effect of toosendanin on tumor growth in nude mice bearing ovarian cancer and its mechanism.Methods Nude mice were randomly divided into groups,and ovarian cancer cells were transplanted for modeling.Cyclophosphamide 0.4 mL/d(10 g/L)was injected intraperitoneally in positive group.In the experimental groups 1,2 and 3,toosendanin 1,10 and 20 mg/kg were dissolved in 0.02 mL/g normal saline for intraperitoneal injection once a day for 15 days.The tumor volume,mass and HE staining results were compared after treatment.Apoptotic indexes were detected by TUNEL method.The expressions of P53,Bax,Bcl-2 and caspase-9 were detected by RT-PCR.The expressions of P53,Bax,Bcl-2 and caspase-9 were detected by Western-blot method.Results Tumor volume and mass were the highest in the model group(P<0.05),and apoptotic index was the lowest(P<0.05).Tumor tissue in the model group had high cell density,disordered arrangement,hyperchromatic nuclei and obvious atypia.In experiment 1,2,positive group and experiment 3 group,the cells were loosely arranged,the size of tumor cells was different,the nuclei were light stained and the nucleoli weresmall.Flake necrosis and apoptotic cells could be found in the tumors.The differences in the expression of Bax,Bcl-2 and caspase-9 and the expression of p-P53/P53 were statistically significant(P<0.05).The differences between the experimental group and the positive group were similar,and the differences between the other two groups were statistically significant(P<0.05).Conclusions Toosendanin inhibits the growth of ovarian cancer in nude mice and promotes the apoptosis of tumor cells.

关 键 词：川楝素 卵巢癌 肿瘤 抑制作用 机制 

分 类 号：R737.31[医药卫生—肿瘤]