饲养层细胞作用机制研究进展  

Research progress on the effects of feeder cells and its mechanism

作　　者：周芳颖 刘玉琴[1] ZHOU Fang-ying;LIU Yu-qin(Department of Pathology,Institute of Basic Medicine,CAMS & PUMC,Beijing 100005,China)

机构地区：[1]中国医学科学院北京协和医学院基础医学研究所病理系

出　　处：《基础医学与临床》2019年第7期1066-1070,共5页Basic and Clinical Medicine

基　　金：国家科技基础条件平台(NSTI-CR18)

摘　　要：饲养层细胞法被广泛应用于干细胞及其他哺乳动物细胞的体外培养,通过分泌生长因子和物理支持可以一定程度模拟细胞增殖的体内环境。根据培养目的的不同,可以有选择性的挑选饲养层细胞种类、处理方法,或通过基因编辑对饲养层细胞进行改良,从而达到最佳的培养效果。Feeder cells have been widely used as an assistant method for the in vitro growth of mammal cells.It can simulate the condition of in vivo culture to a certain extent by the secretion of growth factors and the physical support.According to different culture purposes,we may choose specific feeder cells and dealing methods or modify feeder cells through gene editing to obtain the best culture results.

关 键 词：饲养层细胞 基因修饰 干细胞 诱导多能干细胞 

分 类 号：R73[医药卫生—肿瘤]