超高效合相色谱-四极杆飞行时间质谱联用技术快速分析广西莪术化学成分  

Rapid Characterization of Chemical Constituents in Curcuma Kwangsiensis by Ultra-performance Convergence Chromatography Coupled with Quadrupole Time-of-Flight Mass Spectrometry

作　　者：韦明思[1] 谷筱玉[1] 袁铭 王晖 黄天述[1] 刘佳琪 申玲玲 谭晓杰 WEI Ming-Si;GU Xiao-Yu;YUAN Ming;WANG Hui;HUANG Tian-Shu;LIU Jia-Qi;SHEN Ling-Ling;TAN Xiao-Jie(Guangxi Zhuang Autonomous Region Botanical Garden of Medicinal Plants, Nanning 530023, China;Waters Technologies (Shanghai) Limited Company, Shanghai 201206, China;Shimadzu (China) Corporation, Guangzhou 510010, China)

机构地区：[1]广西壮族自治区药用植物园,南宁530023 [2]沃特世科技(上海)有限公司,上海201206 [3]岛津企业管理(中国)有限公司广州分公司,广州510010

出　　处：《分析化学》2019年第7期1114-1120,共7页Chinese Journal of Analytical Chemistry

基　　金：广西科学研究与技术开发计划项目(No.桂科能14123006-10)资助~~

摘　　要：采用超临界流体萃取(SFE)技术提取广西莪术根茎,应用超高效合相色谱-四极杆飞行时间质谱联用技术(UPC2-QTOF-MS)分析其提取物,结合UNIFI软件的内置中药数据库和自建的姜黄属植物化学成分数据库筛查,快速分离鉴定广西莪术SFE提取物中的化学成分。本研究初步鉴定出广西莪术SFE提取物中47种化合物,其中42种是倍半萜类化合物。47种化合物中有8组同分异构体化合物,共37种化合物。UPC2采用Torus Diol 130色谱柱分离,流动相A为CO2,流动相B为甲醇/异丙醇(3∶1,V/V)+0.1%甲酸,进行梯度洗脱,在7min内实现了47种化合物的分离。本方法无需复杂的样品前处理过程,分析速度快、试剂用量少、成本低、环保,是一种全面高效的分析方法,为中药的化学成分分析提供了新方法。Rapid identification of chemical constituents responsible for defined bioactivities from traditional Chinese medicines (TCMs) is a great challenge. A strategy for rapid identification of the chemical ingredients from the extracts of the rhizoma of Curcuma kwangsiensis with supercritical fluid extraction (SFE) was proposed using ultra-performance convergence chromatography coupled with a quadrupole time-of-flight mass spectrometry (UPC 2-QTOF-MS) combined with UNIFI library. In this study, a total of 47 chemical compounds which were separated in 7 min were tentatively identified, including 42 sesquiterpenes. There were 37 chemical constituents in 8 groups of isomers in the analysis. The strategy which can rapidly, effectively, and comprehensively screen and identify components from TCMs has potential application in TCMs analysis as an alternative method besides UPLC-QTOF-MS and GC-MS.

关 键 词：超高效合相色谱-四极杆飞行时间质谱联用 广西莪术 同分异构体 超临界流体萃取 

分 类 号：O657.63[理学—分析化学]