不同方法测定舒脑欣滴丸中藁本内酯含量的比较  

Comparison of different methods for determination of ligusticide in Shu Naoxin dripping pills

作　　者：高召军 于新祥 范珊 耿亚群 齐永秀 GAO Zhao-jun;YU Xin-xiang;FAN Shan;GENG Ya-qun;QI Yong-xiu(Shandong First Medical University & Shandong Academy of Medical Sciences,Taian 271016,China)

机构地区：[1]山东第一医科大学(山东省医学科学院)

出　　处：《泰山医学院学报》2019年第7期521-523,共3页Journal of Taishan Medical College

基　　金：国家级大学生创新创业训练计划项目(20161043919)

摘　　要：目的比较高效液相色谱法(HPLC)和气相色谱法(GC)测定舒脑欣滴丸中藁本内酯含量的差别。方法采用HPLC法、GC法对舒脑欣滴丸中藁本内酯的含量进行测定。结果GC法,藁本内酯在10~50μL·mL^-1浓度范围内呈现良好的线性关系(r=0.9993),测得的舒脑欣滴丸中藁本内酯含量为676.7μg/丸;HPLC法,藁本内酯浓度在5~50μg·mL^-1浓度范围内与峰面积线性关系良好(r=0.9997),测得的舒脑欣滴丸中藁本内酯含量为687.16μg/丸。结论经F检验两种方法测得舒脑欣滴丸中藁本内酯含量结果基本接近,两种方法均可用作藁本内酯含量测定。Objective:To compare the content of ligustilide in Shunaoxin dropping pills by high performance liquid chromatography(HPLC)and gas chromatography(GC).Methods:HPLC and GC were used to determine the content of ligusticide in Shunaoxin dripping pills.Results:GC showed that ligustilide had a good linear relationship(r=0.9993)in the concentration range of 10~50μL·mL^-1.The content of ligustilide in Shunaoxin dropping pills was 676.7μg/pill.HPLC showed that ligustilide had a good linear relationship with peak area(r=0.9997)in the range of 5~50μg·mL^-1.The content of ligusticide in Shunaoxin dripping pills was 687.16μg/pills.Conclusion:The content of ligustilide in Shunaoxin dropping pills determined by F-test gas chromatography and high performance liquid chromatography is almost the same.Both methods can be used for the determination of ligustilide.

关 键 词：舒脑欣滴丸 藁本内酯 HPLC GC 

分 类 号：R917[医药卫生—药物分析学]