桑枝多酚的制备与其抗氧化活性成分研究  

Preparation and Antioxidant Activities of Polyphenols in Twigs of Morus alba

作　　者：刘鑫彤 孙登阳 叶世芸[1] 唐崇明 曹俊东 周胜[2] 刘江云[2] LIU Xin-tong;SUN Deng-yang;YE Shi-yun;TANG Chong-ming;CAO Jun-dong;ZHOU Sheng;LIU Jiang-yun(Guiyang University of Chinese Medicine,Guiyang 550002,China;College of Pharmaceutical Sciences,SooChow University,Suzhou 215123,China;Pharmacy Department,the First People's Hospital of Yunnan Province,Kunming 650032,China)

机构地区：[1]贵阳中医学院,贵州贵阳550002 [2]苏州大学医学部药学院中药系,江苏苏州215123 [3]云南省第一人民医院药学部,云南昆明650032

出　　处：《中国现代中药》2019年第6期806-810,共5页Modern Chinese Medicine

基　　金：贵州省国内一流建设学科(中药学)(GNYL[2017]008)

摘　　要：目的:对桑枝多酚及其中主要成分的制备工艺和抗氧化活性进行研究,为其开发利用提供参考。方法:桑枝的75%乙醇提取物采用大孔树脂精制,对其中的主成分进行分离鉴定和HPLC含量测定,采用DPPH和ABTS法测试其抗氧化活性。结果:桑枝经提取和精制获得柘木多酚MAP-1~3;从MAP-2、MAP-3中进一步分离获得化合物1、2,经鉴定依次为桑皮苷A、桑皮黄素。采用静态和动态实验筛选,获得优选的AB-8大孔树脂精制工艺参数。抗氧化活性测验结果表明,CTP-1~3对DPPH和ABTS自由基均有抗氧化活性。结论:桑枝多酚具有较强抗氧化活性,桑皮苷A和桑皮黄素是其主要活性成分。Objective: Polyphenols and key ingredients of twigs of Morus alba were investigated for their potential future utilities in this study,together with their antioxidant capacities. Methods: The twigs of Morus alba were extracted with 75% ethanol to give MAP-1,followed by AB-8 macroporous resin purification to give two fractions from 40% and 90% ethanol elution,MAP-2 and MAP-3,respectively. Results: Mulberroside A ( 1 )and mulberrin ( 2 )were separated and identified from MAP-2 and MAP-3,respectively,which were determined to be major ingredients.Both static and dynamic tests were conducted to develop process parameters of MAPs by optimal AB-8 resin.CTP-1-3 showed apparent antioxidant capacities against both DPPH and ABTS free radicals. Conclusion: Polyphenols of twigs of M.alba possess strong antioxidant capacities,with mulberroside A and mulberrin as the major ingredient within them.

关 键 词：桑枝 多酚 桑皮苷A 桑皮黄素 大孔树脂 抗氧化 

分 类 号：R284[医药卫生—中药学]