3种植骨材料在大鼠下颌骨骨缺损修复实验中的长期效果观察  

Observation of long experimental results of three bone implant materials on repair of mandibular defects in rats

作　　者：白玉龙 赵彦涛[1] 沈亚俊 李矛[3] 胡先同 高玉凤 张玥 韩丽伟 衷鸿宾[1] BAI Yu-long;ZHAO Yan-tao;SHEN Ya-jun;LI Mao;HU Xian-tong;GAO Yu-feng;ZHANG Yue;HAN Li-wei;ZHONG Hong-bin(Department of Orthopedics,First Affiliated Hospital of the General Hospital of PLA,Beijing 100048,China)

机构地区：[1]解放军总医院第一附属医院骨科北京100048 [2]解放军第307医院口腔科 [3]解放军第306医院骨科 [4]北京市骨科植入医疗器械工程技术研究中心

出　　处：《中国骨与关节损伤杂志》2019年第6期581-584,共4页Chinese Journal of Bone and Joint Injury

基　　金：国家自然科学基金(81672130);创新环境与平台建设(Z171100002217013);军事医疗重点项目(AWS14C007)

摘　　要：目的评价3种植骨材料在植入大鼠下颌骨缺损模型1年后的成骨作用及降解情况。方法SEM、XRD观察分析材料的结构和组成。选用18只成年SD大鼠,随机分为3组,单侧造直径5 mm的下颌骨全层缺损。A组植入自制煅烧骨,B组植入Bio-Oss骨粉,C组植入Hongros-HA。结果材料结构及组成:A、B组保留了松质骨天然多孔结构,C组呈大小不一的颗粒状,无孔隙结构。3种材料成分均为HA,B组晶体结晶度低于A、C组。术后12个月,所有动物活动良好,取材时皮肤、肌肉形态完好,未见组织坏死。成骨能力:Micro-CT显示,新骨生成率(BV/TV)A组与B组相比差异无统计学意义(P>0.05),均优于C组(P<0.05)。组织学染色可见A、B组缺损大部分被修复,新骨长入较多,C组成骨情况则略差。Lane-Sandhu组织学评分A、B组差异无统计学意义(P>0.05),均优于C组(P<0.05)。降解性能:Micro-CT结果显示,剩余材料占缺损区域的体积百分比(MV/TV)A组与B组相当(P>0.05),均低于C组(P<0.05),HE染色结果也可看到C组材料剩余较多。结论通过低温梯度煅烧工艺制备的煅烧骨材料具有与商品化Bio-Oss相似的结构、成分、成骨潜能及降解性能,为国产异种植骨材料的发展奠定了研究基础。Objective To evaluate the osteogenesis and degradability of three bone materials in rat mandibular defect model for 1 year. Methods The structure and composition of the materials were observed by SEM and XRD. Eighteen adult Sprague-Dawley rats were randomly divided into three groups, a full-length bone defect of the mandible with a diameter of 5 mm was made unilaterally. In group A, self-calcined bone granules were implanted and Bio-Oss were implanted in group B, and group C was implanted with Hongros -HA. Results Material structure and composition: Groups A and B retained the natural porous structure of cancellous bone, and group C showed granular and non-porous structures of different sizes. The three materials were all HA, and the crystallinity of group B was lower than that of groups A and C. At 12 months after operation, all animals were in good activity. The skin and muscles were intact when taken, and no tissue necrosis was observed. Osteogenic capacity: Micro-CT showed that there was no significant difference in the rate of new bone formation (BV/TV) between group A and group B (P >0.05), which was better than group C (P <0.05). Histological staining showed that most of the defects in group A and group B were repaired, new bones grew more, and C component bones were slightly worse. There was no significant difference in the histological scores of Lane-Sandhu between group A and group B (P >0.05), which were better than group C (P <0.05). Degradation performance: Micro-CT results showed that the remaining material accounted for the volume percentage of the defect area (MV/TV). Group A was comparable to group B (P >0.05), both were lower than group C (P <0.05), and HE staining results were also available. More materials in group C remained. Conclusion The calcined bone material prepared by the low temperature gradient calcination process has similar structure, composition, osteogenic potential and degradation performance to commercial Bio-Oss, and lays a research foundation for the development of domestic h

关 键 词：煅烧骨 BIO-OSS Hongros-HA 下颌骨缺损 

分 类 号：R783.1[医药卫生—口腔医学]